Detailed information of TA system TA046626

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3684601..3685358
• Replicon: chromosome
• Accession: NZ_CP007557
• Organism: Citrobacter freundii CFNIH1

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: CFNIH1_RS17525
• Protein ID: WP_080700407.1
• Coordinates: 3684601..3685083 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A2Z3X7E0
• Locus tag: CFNIH1_RS17530
• Protein ID: WP_038640582.1
• Coordinates: 3685074..3685358 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CFNIH1_RS28045	3680537..3680719	+	183	WP_038640578.1	hypothetical protein	-
CFNIH1_RS17515	3680925..3683234	+	2310	WP_038640580.1	N-acetylmuramidase family protein	-
CFNIH1_RS17520	3683680..3684276	-	597	Protein_3453	ISNCY family transposase	-
CFNIH1_RS17525	3684601..3685083	-	483	WP_080700407.1	GNAT family N-acetyltransferase	Toxin
CFNIH1_RS17530	3685074..3685358	-	285	WP_038640582.1	DUF1778 domain-containing protein	Antitoxin
CFNIH1_RS17535	3685613..3685900	+	288	WP_038640585.1	VF530 family protein	-
CFNIH1_RS17540	3685913..3687280	+	1368	WP_038640587.1	glycoside hydrolase family 10 protein	-
CFNIH1_RS17545	3687318..3688589	-	1272	WP_038640589.1	type II toxin-antitoxin system HipA family toxin	-
CFNIH1_RS17550	3688582..3688896	-	315	WP_038640591.1	helix-turn-helix transcriptional regulator	-
CFNIH1_RS25935	3689058..3689570	-	513	WP_051594517.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3679279..3680439	1160

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (31-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (13-87)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17813.46 Da
• Isoelectric Point: 7.2893

>T46626 WP_080700407.1 NZ_CP007557:c3685083-3684601 [Citrobacter freundii CFNIH1]
MEINVTTPVLLTEGHDLQPFDCGNDVLNDWLRRRAIKNQYLNASRTFVICLESTQRVVGYYSIATGSVSHADLGRSLRQN
MPDPVPVVLLGRLAVDVCTQGHSFGKWLLNDAVLRISNLADQVGIKAIMVHAIDGQARAFYEYFGFVQSPIAANTLFYKI

Nucleotide

• Length: 483 bp

>T46626 NZ_CP007557:c3685083-3684601 [Citrobacter freundii CFNIH1]
ATGGAAATAAACGTCACGACACCGGTGTTGCTGACCGAAGGGCATGATCTCCAGCCATTTGATTGCGGTAATGACGTATT
AAATGATTGGCTACGTCGACGGGCGATTAAAAATCAGTACCTCAACGCATCGCGCACATTCGTCATTTGTCTTGAATCTA
CTCAGCGTGTTGTGGGTTACTATTCCATTGCAACCGGGTCCGTGAGCCATGCTGATTTAGGCCGTAGTTTACGTCAGAAT
ATGCCCGACCCGGTTCCGGTCGTTCTGCTGGGACGTCTTGCTGTTGATGTCTGTACTCAGGGGCATAGTTTCGGAAAATG
GCTGCTTAATGATGCGGTACTGCGAATTTCCAACTTAGCCGATCAAGTGGGCATCAAAGCCATCATGGTACATGCTATTG
ATGGCCAAGCTCGGGCATTCTATGAGTATTTCGGCTTCGTTCAGTCTCCGATAGCAGCAAATACGTTGTTCTACAAAATT
TAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10998.45 Da
• Isoelectric Point: 7.0152

>AT46626 WP_038640582.1 NZ_CP007557:c3685358-3685074 [Citrobacter freundii CFNIH1]
MQSSARKPTRDKQINIRATDEERAVIDYAASLVNKNRTDFIIEKAVHEAQNIILDQRVFVLDDARYQSFIEQLEAPVQNV
EGRQRLMNVKPEWK

Nucleotide

• Length: 285 bp

>AT46626 NZ_CP007557:c3685358-3685074 [Citrobacter freundii CFNIH1]
ATGCAAAGCAGCGCCCGTAAGCCGACTCGTGATAAGCAAATTAATATCCGGGCTACTGATGAGGAACGTGCGGTAATTGA
CTACGCTGCAAGTTTGGTCAATAAAAACCGTACTGACTTCATCATTGAGAAGGCCGTGCACGAGGCCCAGAATATCATTT
TAGATCAACGAGTATTCGTCCTTGACGATGCCCGTTATCAGTCTTTCATTGAACAGCTTGAAGCGCCGGTTCAGAACGTT
GAAGGGCGTCAACGCCTGATGAATGTGAAGCCCGAATGGAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A2Z3X7E0