Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2590523..2591143 Replicon chromosome
Accession NZ_CP007557
Organism Citrobacter freundii CFNIH1

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag CFNIH1_RS12285 Protein ID WP_002892050.1
Coordinates 2590523..2590741 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag CFNIH1_RS12290 Protein ID WP_003021733.1
Coordinates 2590769..2591143 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CFNIH1_RS12255 2585882..2586511 + 630 WP_038638321.1 membrane protein -
CFNIH1_RS12260 2586576..2586929 + 354 WP_038638323.1 DUF1428 family protein -
CFNIH1_RS12265 2586981..2588534 - 1554 WP_038638327.1 EAL domain-containing protein -
CFNIH1_RS12270 2588723..2588983 + 261 WP_038638331.1 type B 50S ribosomal protein L31 -
CFNIH1_RS26700 2588985..2589125 + 141 WP_005125190.1 type B 50S ribosomal protein L36 -
CFNIH1_RS12275 2589199..2589669 - 471 WP_038638334.1 YlaC family protein -
CFNIH1_RS12280 2589786..2590337 - 552 WP_038638337.1 maltose O-acetyltransferase -
CFNIH1_RS12285 2590523..2590741 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
CFNIH1_RS12290 2590769..2591143 - 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
CFNIH1_RS12295 2591631..2594780 - 3150 WP_038638345.1 multidrug efflux RND transporter permease subunit -
CFNIH1_RS12300 2594803..2595996 - 1194 WP_038638348.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T46624 WP_002892050.1 NZ_CP007557:c2590741-2590523 [Citrobacter freundii CFNIH1]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T46624 NZ_CP007557:c2590741-2590523 [Citrobacter freundii CFNIH1]
ATGTCTGATAAACCATTAACTAAAACAGATTATTTGATGCGCCTGCGACGCTGCCAGACAATTGACACGCTTGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCTGTATTTTACTCAGCAGCGGATCACCGTCTTGCTGAAT
TGACCATGAATAAGCTCTACGACAAGATTCCAACTTCCGTTTGGAAATTCATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT46624 WP_003021733.1 NZ_CP007557:c2591143-2590769 [Citrobacter freundii CFNIH1]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT46624 NZ_CP007557:c2591143-2590769 [Citrobacter freundii CFNIH1]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAATTTCTCTGCGAAACTCTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGTAACCATGGCTGGGTTAACGACCCTACCTCTGCAACCAGCTTGCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGATTGCGCAGATAGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGTATTAATACGCAGGATCTGCAGAAATGGCGGAAATCAGGTAATCGACT
GTTTCGCTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References