Detailed information of TA system TA046623

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1911688..1912391
• Replicon: chromosome
• Accession: NZ_CP007557
• Organism: Citrobacter freundii CFNIH1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: CFNIH1_RS09110
• Protein ID: WP_038636858.1
• Coordinates: 1912071..1912391 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: CFNIH1_RS26580
• Protein ID: WP_104010294.1
• Coordinates: 1911688..1912029 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CFNIH1_RS09080	1908028..1908462	+	435	WP_038636843.1	hypothetical protein	-
CFNIH1_RS09085	1908482..1909285	+	804	WP_038636846.1	hypothetical protein	-
CFNIH1_RS09090	1909502..1909978	+	477	WP_080700396.1	hypothetical protein	-
CFNIH1_RS09095	1910050..1910871	+	822	WP_038636852.1	DUF945 domain-containing protein	-
CFNIH1_RS09100	1910941..1911411	+	471	WP_038636854.1	DNA repair protein RadC	-
CFNIH1_RS09105	1911420..1911641	+	222	WP_038636856.1	DUF987 family protein	-
CFNIH1_RS26580	1911688..1912029	+	342	WP_104010294.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CFNIH1_RS09110	1912071..1912391	+	321	WP_038636858.1	TA system toxin CbtA family protein	Toxin
CFNIH1_RS09115	1912505..1913341	+	837	WP_038636861.1	DUF4942 domain-containing protein	-
CFNIH1_RS09120	1913711..1914565	-	855	WP_038636864.1	GIY-YIG nuclease family protein	-
CFNIH1_RS09125	1914907..1915089	-	183	WP_038636867.1	hypothetical protein	-
CFNIH1_RS09130	1915835..1917205	+	1371	WP_038636869.1	tyrosine phenol-lyase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (12-105)

Antitoxin

• Antitoxin CbeA (13-112)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12098.86 Da
• Isoelectric Point: 6.3209

>T46623 WP_038636858.1 NZ_CP007557:1912071-1912391 [Citrobacter freundii CFNIH1]
MHISTVPVTVPVSSRLSPVQIWQQLLTYLLEHHYGLTLNDTPFHDDSAIQEHIEVGITLADAVNFLVERYELIRSDRKGF
TWQGQTPFLTAIDILRARRATGLMNI

Nucleotide

• Length: 321 bp

>T46623 NZ_CP007557:1912071-1912391 [Citrobacter freundii CFNIH1]
ATGCATATTTCAACTGTACCGGTCACGGTGCCAGTTTCGTCACGCCTGTCGCCCGTGCAGATCTGGCAGCAACTATTAAC
TTATCTGCTGGAACACCATTACGGTCTAACGCTAAACGACACACCATTCCATGATGATTCCGCCATCCAGGAACATATCG
AAGTGGGAATAACACTGGCTGATGCGGTGAACTTTCTGGTGGAACGCTATGAACTGATACGCTCTGACCGCAAAGGGTTT
ACATGGCAAGGTCAGACACCATTTCTGACCGCTATCGATATTCTCAGAGCCAGGCGAGCTACCGGATTAATGAATATATA
A

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12577.05 Da
• Isoelectric Point: 5.5222

>AT46623 WP_104010294.1 NZ_CP007557:1911688-1912029 [Citrobacter freundii CFNIH1]
ISGTSAEDTSCLQWGLKRNITPCFSARLVQEGNRLYFLADRAGFNSSFSDDEALRLDQAFPLIMKQLERMLTTGELDPRS
QHSVTLHHNGLTSEADTLGSHGYVYIAIYPSQP

Nucleotide

• Length: 342 bp

>AT46623 NZ_CP007557:1911688-1912029 [Citrobacter freundii CFNIH1]
ATATCCGGCACATCGGCGGAGGACACATCCTGCCTTCAGTGGGGATTAAAACGTAATATCACGCCGTGCTTCAGCGCTCG
CCTGGTACAGGAAGGCAACCGCCTATACTTTCTGGCTGACCGGGCGGGGTTTAATAGCTCATTCAGTGACGATGAAGCAT
TGCGCCTAGATCAGGCGTTTCCACTAATAATGAAACAGCTAGAGCGGATGCTCACCACTGGAGAGCTTGATCCCCGCAGT
CAGCACTCCGTCACTCTGCATCACAACGGACTCACTAGTGAAGCTGACACTCTCGGCTCTCATGGCTACGTTTACATCGC
TATTTACCCCAGTCAGCCGTAA