Detailed information of TA system TA046618

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 428506..429345
• Replicon: chromosome
• Accession: NZ_CP007557
• Organism: Citrobacter freundii CFNIH1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A2Z3WYV4
• Locus tag: CFNIH1_RS02070
• Protein ID: WP_038633771.1
• Coordinates: 428968..429345 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: CFNIH1_RS02065
• Protein ID: WP_077249796.1
• Coordinates: 428506..428919 (+)
• Length: 138 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CFNIH1_RS02025	423597..424484	+	888	WP_038633742.1	50S ribosome-binding GTPase	-
CFNIH1_RS02030	424690..425091	+	402	WP_000819262.1	hypothetical protein	-
CFNIH1_RS02035	425188..425655	+	468	WP_000727137.1	hypothetical protein	-
CFNIH1_RS26140	425846..426096	+	251	Protein_399	DUF905 family protein	-
CFNIH1_RS02040	426212..427030	+	819	WP_001234374.1	DUF945 domain-containing protein	-
CFNIH1_RS02045	427030..427293	+	264	WP_038633754.1	hypothetical protein	-
CFNIH1_RS02050	427371..427766	+	396	WP_051594539.1	antirestriction protein	-
CFNIH1_RS02055	427819..428304	+	486	WP_038642868.1	DNA repair protein RadC	-
CFNIH1_RS02060	428316..428537	+	222	WP_038633763.1	DUF987 domain-containing protein	-
CFNIH1_RS02065	428506..428919	+	414	WP_077249796.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CFNIH1_RS02070	428968..429345	+	378	WP_038633771.1	TA system toxin CbtA family protein	Toxin
CFNIH1_RS02075	429342..429833	+	492	WP_038633774.1	hypothetical protein	-
CFNIH1_RS02080	429863..430057	+	195	WP_038633777.1	DUF957 domain-containing protein	-
CFNIH1_RS02085	430138..430987	+	850	Protein_409	DUF4942 domain-containing protein	-
CFNIH1_RS02090	431415..431621	-	207	WP_038633780.1	helix-turn-helix domain-containing protein	-
CFNIH1_RS02095	431618..432079	-	462	Protein_411	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	419538..443301	23763

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (33-132)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14050.89 Da
• Isoelectric Point: 6.8604

>T46618 WP_038633771.1 NZ_CP007557:428968-429345 [Citrobacter freundii CFNIH1]
MQTQPVSPAREASPRLSPVEIWQRLLSHLLDHHYGLALNDTPFGNDDVIQEHIDAGISLCDAVNFIVEKYDLVRTDRYGS
STTEQSPLISSIDILRARKACGLMTRNGYRTVTDITTGKYSKMTR

Nucleotide

• Length: 378 bp

>T46618 NZ_CP007557:428968-429345 [Citrobacter freundii CFNIH1]
ATGCAAACACAACCCGTATCCCCGGCACGGGAGGCTTCTCCACGTCTGTCACCCGTGGAGATTTGGCAGCGACTTCTGAG
CCACCTGCTGGATCATCACTACGGTCTGGCGCTAAACGACACGCCGTTTGGCAACGACGACGTGATTCAGGAGCACATTG
ACGCCGGTATTTCCCTGTGTGATGCCGTCAATTTTATCGTCGAAAAATACGATCTGGTACGCACTGACCGTTACGGATCC
AGCACTACAGAGCAGTCACCGCTTATCAGCAGTATCGATATTCTCCGCGCCCGTAAGGCCTGTGGACTGATGACACGTAA
CGGCTACAGAACGGTGACAGATATCACTACAGGCAAATACAGCAAGATGACCCGATGA

Antitoxin

Protein

• Length: 138 a.a.
• Molecular weight: 15178.32 Da
• Isoelectric Point: 7.7885

>AT46618 WP_077249796.1 NZ_CP007557:428506-428919 [Citrobacter freundii CFNIH1]
MSFCAASPSVNSYSRSTTLLNKTLPANHHISEPWWGLKRNITPCFGARLVQEGNRLHYLADRASITGTFTDADLRHLDQA
FPVLLKQMELMLVSGELSPRHQHCVTLYAKGLTCEADSLGSHGYIYIAIYPTPSVTS

Nucleotide

• Length: 414 bp

>AT46618 NZ_CP007557:428506-428919 [Citrobacter freundii CFNIH1]
ATGTCGTTCTGCGCAGCGTCACCCTCAGTTAACTCATATTCAAGGAGCACGACCTTGTTGAATAAAACACTGCCGGCAAA
TCATCATATTTCTGAACCGTGGTGGGGACTGAAACGTAATATCACCCCGTGCTTCGGTGCACGGCTGGTACAGGAAGGCA
ACCGGCTGCATTATCTGGCCGACCGCGCCAGCATCACCGGGACATTCACCGACGCGGATTTGCGCCATCTCGACCAAGCG
TTTCCGGTACTGCTGAAGCAAATGGAACTGATGCTGGTTTCCGGCGAACTCAGTCCCCGCCATCAGCACTGCGTCACGCT
GTATGCAAAGGGACTGACCTGCGAGGCTGACTCACTCGGATCGCATGGCTATATCTATATAGCGATTTATCCGACACCAT
CAGTAACGTCATAA

Structures

Toxin

• AlphaFold DB: A0A2Z3WYV4