Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 306793..307447 Replicon chromosome
Accession NZ_CP007557
Organism Citrobacter freundii CFNIH1

Toxin (Protein)


Gene name cptA Uniprot ID A0A2Z3X2B8
Locus tag CFNIH1_RS01445 Protein ID WP_038633481.1
Coordinates 306793..307200 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID R8WMJ6
Locus tag CFNIH1_RS01450 Protein ID WP_016154349.1
Coordinates 307181..307447 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CFNIH1_RS01425 302685..304418 - 1734 WP_038633470.1 single-stranded-DNA-specific exonuclease RecJ -
CFNIH1_RS01430 304424..305137 - 714 WP_038633473.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CFNIH1_RS01435 305161..306057 - 897 WP_038633475.1 site-specific tyrosine recombinase XerD -
CFNIH1_RS01440 306171..306692 + 522 WP_038633478.1 flavodoxin FldB -
CFNIH1_RS01445 306793..307200 - 408 WP_038633481.1 protein YgfX Toxin
CFNIH1_RS01450 307181..307447 - 267 WP_016154349.1 FAD assembly factor SdhE Antitoxin
CFNIH1_RS01455 307704..308684 + 981 WP_038633486.1 tRNA-modifying protein YgfZ -
CFNIH1_RS01460 308779..309438 - 660 WP_038633489.1 hemolysin III family protein -
CFNIH1_RS01465 309602..309913 - 312 WP_038633492.1 N(4)-acetylcytidine aminohydrolase -
CFNIH1_RS01470 309966..310694 + 729 WP_038633495.1 MurR/RpiR family transcriptional regulator -
CFNIH1_RS01475 310815..312248 + 1434 WP_038633498.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15944.81 Da        Isoelectric Point: 11.2845

>T46617 WP_038633481.1 NZ_CP007557:c307200-306793 [Citrobacter freundii CFNIH1]
VVQWQSDLRVSWRAQWLSLLVHGLVAVFILLMPWPLSYTPLWMVLLSLVVFDSVRSQRRINACQGEIRLLMDGRLRWQGQ
EWTIVSAPWMVKTGMMLRLRSDTGKRQHLWLAADSMDEAEWRDLRRLMLQQAKQG

Download         Length: 408 bp

>T46617 NZ_CP007557:c307200-306793 [Citrobacter freundii CFNIH1]
GTGGTCCAGTGGCAATCTGATTTACGCGTTTCGTGGCGCGCGCAGTGGCTTTCGTTGCTCGTTCATGGGCTGGTCGCGGT
ATTTATTTTATTGATGCCCTGGCCTTTGAGCTACACCCCATTATGGATGGTTCTGCTCTCTCTGGTGGTGTTCGACAGCG
TACGCAGCCAACGTAGAATTAACGCCTGTCAGGGCGAGATTAGGCTGTTGATGGACGGCCGTCTGCGCTGGCAAGGGCAG
GAGTGGACTATCGTTAGCGCGCCCTGGATGGTCAAAACAGGCATGATGCTGCGTTTGCGTTCAGATACCGGGAAACGTCA
GCATTTGTGGCTGGCGGCAGACAGTATGGATGAGGCTGAGTGGCGAGACTTACGGCGGTTAATGCTGCAACAGGCCAAGC
AGGGGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10557.10 Da        Isoelectric Point: 5.6375

>AT46617 WP_016154349.1 NZ_CP007557:c307447-307181 [Citrobacter freundii CFNIH1]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDEEKRIFIRLLECDDPDLFNWLMNHGKPADAQLEQMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT46617 NZ_CP007557:c307447-307181 [Citrobacter freundii CFNIH1]
ATGGATATTAACAATAAAGCACGTATTCATTGGGCATGCCGCCGCGGTATGCGCGAGCTGGACATCTCTATCATGCCGTT
TTTCGAACATGAATATGACAGCTTAAGTGACGAAGAAAAACGTATCTTTATCCGTCTGCTAGAATGTGACGATCCAGATT
TATTTAACTGGTTGATGAATCATGGGAAACCAGCTGATGCTCAGTTGGAGCAGATGGTGCGACTTATCCAGACACGGAAT
CGGGAACGTGGTCCAGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z3X2B8

Antitoxin

Source ID Structure
AlphaFold DB R8WMJ6

References