Detailed information of TA system TA046616

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-RHH
• Location: 3994144..3994760
• Replicon: chromosome
• Accession: NZ_CP007546
• Organism: Enterobacter asburiae L1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: DI57_RS18840
• Protein ID: WP_029741589.1
• Coordinates: 3994389..3994760 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A376FEL2
• Locus tag: DI57_RS18835
• Protein ID: WP_029741590.1
• Coordinates: 3994144..3994386 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DI57_RS18805	3989562..3990032	+	471	WP_029740330.1	hypothetical protein	-
DI57_RS18810	3990121..3990720	+	600	WP_029740331.1	glucose-1-phosphatase	-
DI57_RS18815	3990714..3991595	+	882	WP_029740332.1	virulence factor BrkB family protein	-
DI57_RS18820	3991592..3992029	+	438	WP_008501836.1	D-tyrosyl-tRNA(Tyr) deacylase	-
DI57_RS18825	3992074..3993015	+	942	WP_023309755.1	fatty acid biosynthesis protein FabY	-
DI57_RS18830	3993024..3993944	-	921	WP_038417758.1	alpha/beta hydrolase	-
DI57_RS18835	3994144..3994386	+	243	WP_029741590.1	ribbon-helix-helix domain-containing protein	Antitoxin
DI57_RS18840	3994389..3994760	+	372	WP_029741589.1	type II toxin-antitoxin system VapC family toxin	Toxin
DI57_RS18845	3994800..3995729	-	930	WP_029741588.1	formate dehydrogenase accessory protein FdhE	-
DI57_RS18850	3995726..3996361	-	636	WP_010436852.1	formate dehydrogenase cytochrome b556 subunit	-
DI57_RS18855	3996358..3997260	-	903	WP_008501831.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-109)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13589.71 Da
• Isoelectric Point: 6.9789

>T46616 WP_029741589.1 NZ_CP007546:3994389-3994760 [Enterobacter asburiae L1]
MEHMAVFDTNILIDLFNNRVEAADAIDRTASHRAISLITWMEVMVGARKHGHEAKTAAVMGAFEIIDLSRDIAERSVLLR
EKHGMKLPDAIILATAQSRSCPLISRNTKDFSGITGVVSPYQL

Nucleotide

• Length: 372 bp

>T46616 NZ_CP007546:3994389-3994760 [Enterobacter asburiae L1]
ATGGAGCACATGGCAGTGTTTGATACCAATATTCTCATCGATCTTTTTAATAATCGTGTTGAGGCGGCGGATGCCATTGA
TCGCACGGCGTCGCATAGAGCCATTAGCCTGATCACGTGGATGGAAGTCATGGTGGGTGCGCGCAAGCACGGACATGAAG
CAAAAACGGCGGCTGTGATGGGGGCTTTTGAGATTATTGATCTTTCTCGGGATATCGCAGAAAGAAGCGTTTTACTTCGC
GAGAAACATGGGATGAAACTGCCTGATGCCATTATTCTGGCTACTGCTCAGAGCAGAAGTTGCCCGTTAATCTCACGCAA
TACGAAAGACTTTTCGGGCATTACTGGAGTCGTTTCACCTTACCAGCTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9401.51 Da
• Isoelectric Point: 4.1735

>AT46616 WP_029741590.1 NZ_CP007546:3994144-3994386 [Enterobacter asburiae L1]
MSLSADLDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIEQYLDQQSSSVIRDALGLWGNQQEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT46616 NZ_CP007546:3994144-3994386 [Enterobacter asburiae L1]
ATGAGTCTTTCCGCGGATCTTGATATGGGCAGAATTCTGATCGATTTGTCTGATGACGTAATTCAACGACTGGATAATCT
GAAGCAGCTGCGTAACCAACCTCGTGCCGAATTACTGCGTGAAGCAATTGAGCAATATCTCGACCAGCAGAGTTCATCGG
TTATCCGCGATGCGCTTGGCCTGTGGGGAAACCAGCAGGAAGATGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A376FEL2