Detailed information of TA system TA046602

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 193007..193746
• Replicon: chromosome
• Accession: NZ_CP007546
• Organism: Enterobacter asburiae L1

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: DI57_RS01020
• Protein ID: WP_029739942.1
• Coordinates: 193261..193746 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A157GCY0
• Locus tag: DI57_RS01015
• Protein ID: WP_029739941.1
• Coordinates: 193007..193273 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DI57_RS00995	188191..190035	+	1845	WP_038417274.1	cell envelope integrity protein TolA	-
DI57_RS21675	190100..190558	+	459	WP_155268072.1	hypothetical protein	-
DI57_RS21810	190581..190832	-	252	WP_071841167.1	hypothetical protein	-
DI57_RS01005	191076..191354	+	279	WP_038417276.1	hypothetical protein	-
DI57_RS01010	191384..192562	-	1179	WP_038417279.1	tyrosine-type recombinase/integrase	-
DI57_RS01015	193007..193273	+	267	WP_029739941.1	DUF1778 domain-containing protein	Antitoxin
DI57_RS01020	193261..193746	+	486	WP_029739942.1	GNAT family N-acetyltransferase	Toxin
DI57_RS01025	194156..196066	+	1911	WP_029739943.1	NACHT domain-containing protein	-
DI57_RS01030	196237..196535	+	299	Protein_205	integrase	-
DI57_RS01035	196690..197760	-	1071	WP_029739945.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	179201..196485	17284

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (54-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17681.39 Da
• Isoelectric Point: 9.0539

>T46602 WP_029739942.1 NZ_CP007546:193261-193746 [Enterobacter asburiae L1]
VGRVTAPEPLSSSHQLAEFVSGEAVLDDWLKQRGLKNQALGAARTFVVCKTDTKQVAGFYSLATGSVNQVEATGSLRRNM
PDPIPVIILARLAVDVSFRGKGLEADLLHDAVLRCYRVAENIGVRAIMVYALTEEAKSFYLHHGFKASQIQERTLFLKLP
Q

Nucleotide

• Length: 486 bp

>T46602 NZ_CP007546:193261-193746 [Enterobacter asburiae L1]
GTGGGACGTGTAACAGCACCAGAACCGTTATCCAGTTCTCATCAACTTGCTGAGTTTGTCAGTGGTGAGGCTGTACTTGA
TGACTGGCTTAAACAGAGAGGATTAAAGAATCAGGCTCTCGGTGCCGCCCGAACGTTCGTTGTTTGTAAAACAGACACGA
AACAGGTTGCCGGTTTTTACTCTCTGGCCACGGGCAGCGTTAACCAAGTGGAGGCAACGGGAAGCCTCAGGCGCAATATG
CCAGATCCCATCCCCGTAATCATTCTTGCTCGCCTGGCTGTCGATGTTTCATTCCGTGGAAAAGGGCTCGAGGCTGATTT
ACTGCATGATGCAGTTCTGCGCTGTTACCGTGTAGCTGAGAATATTGGAGTTCGTGCGATTATGGTTTATGCACTGACTG
AAGAAGCCAAAAGCTTTTACCTTCACCATGGCTTTAAGGCATCGCAAATCCAGGAGCGCACATTGTTTCTCAAATTACCA
CAGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10038.42 Da
• Isoelectric Point: 4.4733

>AT46602 WP_029739941.1 NZ_CP007546:193007-193273 [Enterobacter asburiae L1]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVTDDATIGKLL
GRKPQWDV

Nucleotide

• Length: 267 bp

>AT46602 NZ_CP007546:193007-193273 [Enterobacter asburiae L1]
ATGAAATCAGATGTTCAGCTCAACCTTAGAGCTAAGGAATCTCAAAGAGCACTCATTGATGCTGCAGCAGAGATTCTCCA
TAAATCACGCACAGACTTCATCCTGGAAATGGCATGTCAGGCTGCTGAGAATGTGATCCTCGATCGTCGCGTATTTAATT
TTAACGATGAGCAGTATGCAGAGTTTATCGATATGCTCGATGCACCAGTTACGGATGACGCTACCATTGGTAAACTCCTG
GGAAGGAAACCTCAGTGGGACGTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A157GCY0