Detailed information of TA system TA046581

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelB(antitoxin)
• Location: 1675309..1675921
• Replicon: chromosome
• Accession: NZ_CP007537
• Organism: Streptococcus pyogenes strain AP1

Toxin (Protein)

• Gene name: parE
• Uniprot ID: J7M9F1
• Locus tag: SPAP1_RS08665
• Protein ID: WP_010922665.1
• Coordinates: 1675586..1675921 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: Q5X9X9
• Locus tag: SPAP1_RS08660
• Protein ID: WP_002988079.1
• Coordinates: 1675309..1675596 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SPAP1_RS08635	1670486..1671469	-	984	WP_002982755.1	tagatose-bisphosphate aldolase	-
SPAP1_RS08640	1671473..1672402	-	930	WP_010922663.1	tagatose-6-phosphate kinase	-
SPAP1_RS08645	1672450..1672965	-	516	WP_002988082.1	galactose-6-phosphate isomerase subunit LacB	-
SPAP1_RS08650	1673000..1673428	-	429	WP_010922664.1	galactose-6-phosphate isomerase subunit LacA	-
SPAP1_RS08655	1673875..1674648	+	774	WP_002982738.1	DeoR/GlpR family DNA-binding transcription regulator	-
SPAP1_RS08660	1675309..1675596	+	288	WP_002988079.1	DNA-damage-inducible protein J	Antitoxin
SPAP1_RS08665	1675586..1675921	+	336	WP_010922665.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SPAP1_RS10065	1676073..1676255	+	183	WP_011285716.1	integrase	-
SPAP1_RS10070	1676589..1677026	+	438	WP_111713473.1	site-specific integrase	-
SPAP1_RS08675	1677146..1677538	-	393	WP_002982716.1	30S ribosomal protein S9	-
SPAP1_RS08680	1677559..1678005	-	447	WP_002982710.1	50S ribosomal protein L13	-
SPAP1_RS08685	1678223..1678429	-	207	WP_002982695.1	helix-turn-helix transcriptional regulator	-
SPAP1_RS08690	1678426..1678932	-	507	WP_002988070.1	hypothetical protein	-
SPAP1_RS08695	1679068..1679928	-	861	WP_002982687.1	DegV family protein	-
SPAP1_RS08700	1680025..1680543	-	519	WP_002982682.1	NYN domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-105)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 13185.99 Da
• Isoelectric Point: 5.2144

>T46581 WP_010922665.1 NZ_CP007537:1675586-1675921 [Streptococcus pyogenes]
MDYKKYQIIYAPDVLEKLKEIRDYISQNYSSTSGQRKMEQIISDIEKLEVFPEVGFDADEKYGSKIIHYHSTKGYTLSKD
YIVLYHIEGEENRIVIDYLLPTQSDYIKLFK

Nucleotide

• Length: 336 bp

>T46581 NZ_CP007537:1675586-1675921 [Streptococcus pyogenes]
TTGGACTATAAGAAATATCAGATTATCTATGCTCCTGATGTTTTAGAGAAGCTAAAAGAAATTCGTGATTATATTTCTCA
AAACTATTCCTCAACATCAGGTCAGCGTAAAATGGAGCAAATCATTAGCGACATAGAAAAACTCGAGGTTTTCCCAGAAG
TTGGTTTTGATGCCGATGAAAAATATGGTTCAAAAATCATCCATTATCACAGCACTAAAGGTTATACCTTAAGTAAAGAT
TATATTGTCCTATACCATATCGAGGGGGAAGAAAATAGGATCGTTATTGATTACTTGCTTCCTACTCAAAGCGACTATAT
AAAGTTATTCAAATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10934.56 Da
• Isoelectric Point: 5.2318

>AT46581 WP_002988079.1 NZ_CP007537:1675309-1675596 [Streptococcus pyogenes]
MTTVKKNRAVTFQANKELVSEAMTVLNKKNLTLSSALRLFLQNVVVTNEVDLLTEEELEKEKLFKQFQAEINKNIEDVRQ
GKFYTSEEVRAELGL

Nucleotide

• Length: 288 bp

>AT46581 NZ_CP007537:1675309-1675596 [Streptococcus pyogenes]
ATGACTACAGTAAAAAAAAACAGAGCGGTTACCTTTCAAGCTAATAAAGAATTGGTAAGCGAAGCAATGACAGTATTAAA
CAAAAAAAATTTAACCTTATCATCTGCTTTAAGATTATTCCTACAAAATGTCGTTGTCACAAACGAGGTTGACTTATTGA
CGGAAGAGGAGTTAGAGAAAGAAAAACTTTTCAAGCAATTTCAAGCTGAAATCAACAAAAATATTGAGGATGTTCGTCAA
GGGAAGTTTTATACCTCTGAGGAAGTGAGGGCTGAACTTGGACTATAA

Structures

Toxin

• AlphaFold DB: J7M9F1

Antitoxin

• AlphaFold DB: A0A4U7GX94