Detailed information of TA system TA046574

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1391619..1392267
• Replicon: chromosome
• Accession: NZ_CP007514
• Organism: Rubrobacter radiotolerans strain RSPS-4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: RRADSPS_RS06620
• Protein ID: WP_038681531.1
• Coordinates: 1391619..1392029 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A023X2R1
• Locus tag: RRADSPS_RS06625
• Protein ID: WP_038681533.1
• Coordinates: 1392031..1392267 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RRADSPS_RS06595	1386633..1388402	+	1770	WP_198024576.1	glyoxylate carboligase	-
RRADSPS_RS06600	1388448..1389122	-	675	WP_038681527.1	hemolysin III family protein	-
RRADSPS_RS06605	1389235..1389813	+	579	WP_084263788.1	crossover junction endodeoxyribonuclease RuvC	-
RRADSPS_RS06610	1389838..1390458	+	621	WP_038681529.1	Holliday junction branch migration protein RuvA	-
RRADSPS_RS06615	1390481..1391611	+	1131	WP_084264076.1	Holliday junction branch migration DNA helicase RuvB	-
RRADSPS_RS06620	1391619..1392029	-	411	WP_038681531.1	type II toxin-antitoxin system VapC family toxin	Toxin
RRADSPS_RS06625	1392031..1392267	-	237	WP_038681533.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
RRADSPS_RS06630	1392350..1395154	+	2805	WP_051589471.1	class I SAM-dependent DNA methyltransferase	-
RRADSPS_RS06635	1395283..1395498	+	216	WP_038681535.1	hypothetical protein	-
RRADSPS_RS06640	1395554..1396738	+	1185	WP_084263789.1	DUF2183 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-127)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14858.09 Da
• Isoelectric Point: 6.4723

>T46574 WP_038681531.1 NZ_CP007514:c1392029-1391619 [Rubrobacter radiotolerans]
MSQLRRLLDTNVCIALIRARSPHALRRFEDFEPGEVGVSAITVAELRYGVEKSAAVGRNRDALARFLLPLEVLAFDSEAT
ELYGKVRAGLERQGMPIGPLDTLIVAHALSIGTTLVTNNTREFGRVPGLAVEDWTA

Nucleotide

• Length: 411 bp

>T46574 NZ_CP007514:c1392029-1391619 [Rubrobacter radiotolerans]
ATGTCACAGCTCAGGCGACTGCTCGACACAAACGTCTGCATAGCCCTGATCCGGGCCCGCTCCCCGCACGCGCTCCGCCG
GTTCGAGGACTTCGAGCCGGGCGAAGTCGGGGTCTCAGCCATAACCGTCGCGGAGCTTCGCTACGGGGTCGAGAAGAGCG
CGGCGGTCGGCAGGAACCGGGACGCGCTTGCGAGATTCCTCCTGCCGCTGGAGGTCCTGGCCTTCGACTCCGAAGCTACG
GAGCTTTACGGCAAGGTACGAGCCGGGCTCGAAAGACAGGGGATGCCCATAGGTCCGCTGGATACCCTTATAGTCGCCCA
CGCCCTTAGCATCGGTACCACGCTGGTAACGAACAACACCCGGGAGTTTGGTCGTGTCCCGGGCCTCGCTGTCGAAGACT
GGACCGCCTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9037.10 Da
• Isoelectric Point: 4.8151

>AT46574 WP_038681533.1 NZ_CP007514:c1392267-1392031 [Rubrobacter radiotolerans]
VDTAKLFKSGRSQAVRLPKAYRFEGEEVFVKRVGDAVVLLPREDTWRTLYRSLDDFSEDFMQSRDQPPAPDAREPFSD

Nucleotide

• Length: 237 bp

>AT46574 NZ_CP007514:c1392267-1392031 [Rubrobacter radiotolerans]
GTGGATACGGCGAAGCTGTTCAAGAGCGGTCGCAGTCAGGCCGTGCGGCTGCCGAAGGCTTACCGCTTCGAGGGCGAGGA
GGTCTTCGTGAAGCGGGTTGGGGACGCGGTCGTGCTGCTCCCGCGGGAGGACACCTGGCGCACGCTCTACAGGAGCCTCG
ACGACTTCTCCGAGGACTTCATGCAGAGCCGGGACCAGCCTCCCGCACCGGACGCACGCGAGCCGTTCTCCGACTGA

Structures

Antitoxin

• AlphaFold DB: A0A023X2R1