Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 124605..125143 Replicon chromosome
Accession NZ_CP007501
Organism Polynucleobacter duraquae strain MWH-MoK4

Toxin (Protein)


Gene name mazF Uniprot ID A0A0E3UZK9
Locus tag CL55_RS00730 Protein ID WP_046329437.1
Coordinates 124605..124931 (-) Length 109 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A0E3ZK86
Locus tag CL55_RS00735 Protein ID WP_046329438.1
Coordinates 124928..125143 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CL55_RS00705 120630..120938 - 309 WP_046329433.1 Grx4 family monothiol glutaredoxin -
CL55_RS00710 121041..121889 - 849 WP_046329434.1 peptide chain release factor N(5)-glutamine methyltransferase -
CL55_RS00715 121902..122981 - 1080 WP_046329435.1 peptide chain release factor 1 -
CL55_RS00720 122982..124298 - 1317 WP_046329436.1 glutamyl-tRNA reductase -
CL55_RS00725 124342..124608 + 267 WP_205621313.1 hypothetical protein -
CL55_RS00730 124605..124931 - 327 WP_046329437.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
CL55_RS00735 124928..125143 - 216 WP_046329438.1 antitoxin MazE family protein Antitoxin
CL55_RS00740 125215..125985 - 771 WP_046329439.1 uracil-DNA glycosylase -
CL55_RS00745 125985..127769 - 1785 WP_046329440.1 M61 family metallopeptidase -
CL55_RS00750 127782..128507 - 726 WP_205621314.1 DsbC family protein -
CL55_RS00755 128491..129765 - 1275 WP_052728690.1 FAD-dependent monooxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-104)

Antitoxin

Antitoxin MazE-like

(8-69)


Sequences

Toxin        


Download         Length: 109 a.a.        Molecular weight: 11740.71 Da        Isoelectric Point: 8.8085

>T46560 WP_046329437.1 NZ_CP007501:c124931-124605 [Polynucleobacter duraquae]
VKRGNVVTVAMQGDFGKPRPALVLQSDVFSDIHATVTVALISSEIKQAPIFRLDIEPNEANGLTRASQVQIDKIMSIRSE
KIGSVIGELNDVMMVRVNRALALWLGLA

Download         Length: 327 bp

>T46560 NZ_CP007501:c124931-124605 [Polynucleobacter duraquae]
GTGAAGCGTGGCAACGTGGTAACGGTTGCTATGCAGGGTGACTTTGGAAAGCCGAGACCAGCCTTAGTACTTCAATCGGA
CGTTTTCAGTGATATTCATGCAACGGTAACGGTTGCCTTGATATCCAGTGAAATTAAACAAGCTCCAATCTTCCGACTTG
ATATTGAACCCAATGAAGCCAATGGGCTAACCCGAGCATCACAAGTTCAGATTGATAAGATCATGTCAATTCGAAGTGAA
AAAATTGGATCTGTAATTGGCGAGCTCAATGACGTCATGATGGTACGAGTCAACAGAGCCCTAGCGCTTTGGTTAGGCTT
AGCCTAA

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7894.90 Da        Isoelectric Point: 4.4702

>AT46560 WP_046329438.1 NZ_CP007501:c125143-124928 [Polynucleobacter duraquae]
MPSNAALRVQKHRLALRDAGLRPIQIWVPDTQAPGFFEECRRQSALVAAADRADNELMSFLDDSLADLDEA

Download         Length: 216 bp

>AT46560 NZ_CP007501:c125143-124928 [Polynucleobacter duraquae]
ATGCCTTCAAATGCCGCGCTTAGAGTCCAAAAACACCGGCTAGCCCTCAGAGATGCTGGATTACGGCCTATTCAAATATG
GGTTCCAGATACTCAAGCCCCTGGCTTTTTTGAAGAATGTCGTCGCCAATCAGCTCTAGTAGCCGCTGCCGATCGAGCAG
ACAATGAGCTGATGTCCTTTCTCGATGACTCATTAGCAGATTTGGATGAAGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E3UZK9

Antitoxin

Source ID Structure
AlphaFold DB A0A0E3ZK86

References