Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /HTH_XRE(antitoxin)
Location 1153954..1154751 Replicon chromosome
Accession NZ_CP007499
Organism Staphylococcus aureus strain 2395 USA500

Toxin (Protein)


Gene name - Uniprot ID A0A6B0AWK0
Locus tag CH51_RS05765 Protein ID WP_000525016.1
Coordinates 1153954..1154415 (-) Length 154 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A0C2LD36
Locus tag CH51_RS05770 Protein ID WP_001260487.1
Coordinates 1154428..1154751 (-) Length 108 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CH51_RS05735 1149482..1149964 + 483 WP_000401377.1 pantetheine-phosphate adenylyltransferase -
CH51_RS05740 1150026..1151165 - 1140 WP_000843611.1 nucleotidyltransferase -
CH51_RS05745 1151292..1151849 + 558 WP_000872158.1 DUF177 domain-containing protein -
CH51_RS05755 1151929..1152102 + 174 WP_000290472.1 50S ribosomal protein L32 -
CH51_RS05760 1152219..1153604 - 1386 WP_000861313.1 recombinase family protein -
CH51_RS05765 1153954..1154415 - 462 WP_000525016.1 hypothetical protein Toxin
CH51_RS05770 1154428..1154751 - 324 WP_001260487.1 helix-turn-helix domain-containing protein Antitoxin
CH51_RS05775 1154915..1155163 + 249 WP_000272858.1 helix-turn-helix domain-containing protein -
CH51_RS16600 1155178..1155339 + 162 WP_001153175.1 hypothetical protein -
CH51_RS05780 1155332..1155625 - 294 WP_044118938.1 hypothetical protein -
CH51_RS05785 1155633..1156376 + 744 WP_001148623.1 phage antirepressor KilAC domain-containing protein -
CH51_RS05790 1156552..1156782 - 231 WP_000395455.1 hypothetical protein -
CH51_RS05795 1156962..1157123 + 162 WP_000066025.1 DUF1270 domain-containing protein -
CH51_RS05800 1157218..1157544 + 327 WP_000165362.1 DUF2482 family protein -
CH51_RS05805 1157525..1157785 + 261 WP_000291503.1 DUF1108 family protein -
CH51_RS15865 1157794..1158057 + 264 WP_001205732.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1147057..1194046 46989


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(8-65)


Sequences

Toxin        


Download         Length: 154 a.a.        Molecular weight: 17993.29 Da        Isoelectric Point: 4.5941

>T46543 WP_000525016.1 NZ_CP007499:c1154415-1153954 [Staphylococcus aureus]
MGLYEETLIQHDYIEVREADVLPDNLDGVWLGDLILIKRGLSDTEKAGILFEELAHNKLTYGDIADYSKFNNRKFENYAR
RHGFTSAVPIREIVEAYNYGVRNLYELSEYLQLSEEYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI

Download         Length: 462 bp

>T46543 NZ_CP007499:c1154415-1153954 [Staphylococcus aureus]
ATGGGATTATATGAAGAAACTTTAATACAACATGATTATATTGAAGTAAGAGAGGCTGATGTACTTCCAGATAATTTAGA
CGGGGTATGGTTAGGAGATCTAATTTTAATAAAGCGTGGTTTATCAGATACAGAAAAGGCAGGAATTCTCTTCGAAGAAT
TAGCACATAATAAACTTACATACGGTGATATAGCTGATTACTCGAAATTCAACAATCGCAAGTTCGAAAATTATGCACGT
AGACATGGTTTTACTTCAGCTGTACCGATACGTGAAATTGTAGAAGCTTACAATTATGGCGTACGTAACTTGTATGAGTT
GTCTGAGTATCTACAATTAAGCGAAGAATACATATTGGAGGCAATAGAACAATATAAGAAGATATACGGTATTGGAACTC
ACTATGGCGAGTATTCGATTACATTTGAGCCGTTGAGAGTTTTTAAATATAAAGAAATATGA

Antitoxin


Download         Length: 108 a.a.        Molecular weight: 12431.09 Da        Isoelectric Point: 9.3811

>AT46543 WP_001260487.1 NZ_CP007499:c1154751-1154428 [Staphylococcus aureus]
MRTSAEIGKLIKQLRKENNVNLTDFATKIGVNKSTLSRYENGSRKIPMEDIAEIANALKVTPEYLLLKNRQTENEVQHRA
AHLEGELTDDEWQRVLDYADYIRSKRK

Download         Length: 324 bp

>AT46543 NZ_CP007499:c1154751-1154428 [Staphylococcus aureus]
ATGAGAACTTCAGCAGAAATAGGTAAATTAATCAAACAACTACGAAAAGAGAATAATGTGAATTTAACTGATTTTGCAAC
TAAGATAGGTGTCAATAAATCTACCTTATCCCGATATGAAAACGGTAGCAGAAAAATACCTATGGAGGATATAGCTGAAA
TTGCCAATGCATTGAAAGTTACCCCAGAATATTTACTATTAAAAAATAGACAAACAGAAAACGAAGTACAACATCGAGCA
GCTCACCTTGAAGGAGAATTGACAGATGATGAATGGCAAAGAGTTTTAGATTATGCAGATTATATAAGAAGTAAACGTAA
GTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6B0AWK0

Antitoxin

Source ID Structure
AlphaFold DB A0A0C2LD36

References