Detailed information of TA system TA046540

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TscAT/-
• Location: 404968..405494
• Replicon: chromosome
• Accession: NZ_CP007499
• Organism: Staphylococcus aureus strain 2395 USA500

Toxin (Protein)

• Gene name: TscT
• Uniprot ID: -
• Locus tag: CH51_RS01880
• Protein ID: WP_001103949.1
• Coordinates: 405180..405494 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: TscA
• Uniprot ID: X5IY59
• Locus tag: CH51_RS01875
• Protein ID: WP_001058494.1
• Coordinates: 404968..405177 (+)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CH51_RS01835	400088..400591	+	504	WP_000934799.1	single-stranded DNA-binding protein	-
CH51_RS01840	400643..400885	+	243	WP_000897044.1	30S ribosomal protein S18	-
CH51_RS01845	401114..401911	-	798	WP_000119675.1	type II toxin-antitoxin system PemK/MazF family toxin	-
CH51_RS01850	401935..403149	-	1215	WP_000270128.1	site-specific integrase	-
CH51_RS01855	403448..404182	-	735	WP_000142630.1	helix-turn-helix domain-containing protein	-
CH51_RS01860	404332..404544	+	213	WP_001063623.1	helix-turn-helix transcriptional regulator	-
CH51_RS01865	404545..404817	+	273	WP_001138303.1	helix-turn-helix domain-containing protein	-
CH51_RS01870	404829..404975	+	147	WP_000784873.1	hypothetical protein	-
CH51_RS01875	404968..405177	+	210	WP_001058494.1	hypothetical protein	Antitoxin
CH51_RS01880	405180..405494	+	315	WP_001103949.1	DUF1474 family protein	Toxin
CH51_RS01885	405565..407817	+	2253	WP_000974924.1	DNA primase	-
CH51_RS01890	408088..408432	+	345	WP_000567971.1	hypothetical protein	-
CH51_RS01895	408434..409075	+	642	WP_001019274.1	hypothetical protein	-
CH51_RS01900	409525..409866	+	342	WP_001190606.1	hypothetical protein	-
CH51_RS01905	409878..410456	+	579	WP_000846280.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	tet(M)	-	364134..467599	103465

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1474 (1-98)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12374.05 Da
• Isoelectric Point: 6.2243

>T46540 WP_001103949.1 NZ_CP007499:405180-405494 [Staphylococcus aureus]
MNWEIKDLMCDIEVIKQKINDVATKHAWFVKDRFVKNELETKREHINFSASYLEHRIQNEHTVELLHVYLKEFGELIQKF
HEIEKASSENFGEVSDDAKNVKKA

Nucleotide

• Length: 315 bp

>T46540 NZ_CP007499:405180-405494 [Staphylococcus aureus]
ATGAATTGGGAAATTAAAGATTTAATGTGTGACATTGAAGTGATAAAACAAAAAATTAATGATGTAGCTACCAAACATGC
TTGGTTTGTTAAAGATAGATTTGTAAAAAATGAATTAGAAACAAAACGGGAACATATTAATTTTTCTGCTAGCTATTTAG
AACATCGTATACAAAATGAACATACAGTTGAGTTATTACATGTGTACTTAAAAGAATTCGGTGAACTTATACAAAAATTT
CATGAAATAGAAAAAGCATCATCTGAGAACTTTGGCGAGGTATCAGATGACGCAAAGAATGTGAAAAAAGCATAA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 8268.31 Da
• Isoelectric Point: 4.3718

>AT46540 WP_001058494.1 NZ_CP007499:404968-405177 [Staphylococcus aureus]
MNNEQKEVIEHVVYQLELSVMNNLESYEHTEYVNSIEVVSEISREKHLELIMKWCAQELKNNFQLEKGE

Nucleotide

• Length: 210 bp

>AT46540 NZ_CP007499:404968-405177 [Staphylococcus aureus]
ATGAATAATGAACAAAAAGAAGTAATAGAACACGTGGTTTATCAACTTGAGTTAAGTGTCATGAATAACTTGGAAAGTTA
TGAACACACAGAATATGTTAATAGTATTGAAGTGGTTTCAGAGATCAGTCGTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAGAAAGGAGAATAA

Structures

Antitoxin

• AlphaFold DB: X5IY59