Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3098698..3099352 Replicon chromosome
Accession NZ_CP007491
Organism Escherichia coli strain ACN002

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag ACN002_RS15220 Protein ID WP_000244777.1
Coordinates 3098698..3099105 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag ACN002_RS15225 Protein ID WP_000354046.1
Coordinates 3099086..3099352 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ACN002_RS15200 3094655..3096388 - 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -
ACN002_RS15205 3096394..3097104 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
ACN002_RS15210 3097129..3098025 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
ACN002_RS15215 3098137..3098658 + 522 WP_001055874.1 flavodoxin FldB -
ACN002_RS15220 3098698..3099105 - 408 WP_000244777.1 toxin CptA Toxin
ACN002_RS15225 3099086..3099352 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
ACN002_RS15230 3099595..3100575 + 981 WP_000886061.1 tRNA-modifying protein YgfZ -
ACN002_RS15235 3100652..3101311 - 660 WP_000250274.1 hemolysin III family protein -
ACN002_RS15240 3101475..3101786 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
ACN002_RS15245 3101831..3103264 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
ACN002_RS15250 3103321..3104064 - 744 WP_000951948.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T46522 WP_000244777.1 NZ_CP007491:c3099105-3098698 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T46522 NZ_CP007491:c3099105-3098698 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT46522 WP_000354046.1 NZ_CP007491:c3099352-3099086 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT46522 NZ_CP007491:c3099352-3099086 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References