Detailed information of TA system TA046519

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2655870..2656495
• Replicon: chromosome
• Accession: NZ_CP007491
• Organism: Escherichia coli strain ACN002

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: ACN002_RS13030
• Protein ID: WP_000911330.1
• Coordinates: 2655870..2656268 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: ACN002_RS13035
• Protein ID: WP_000450524.1
• Coordinates: 2656268..2656495 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACN002_RS13010	2651748..2651948	+	201	WP_000383836.1	YpfN family protein	-
ACN002_RS13015	2652058..2652756	-	699	WP_000679823.1	esterase	-
ACN002_RS13020	2652830..2654845	-	2016	WP_000829323.1	tRNA cytosine(34) acetyltransferase TmcA	-
ACN002_RS13025	2654860..2655723	-	864	WP_001267507.1	neutral zinc metallopeptidase	-
ACN002_RS13030	2655870..2656268	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
ACN002_RS13035	2656268..2656495	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
ACN002_RS13040	2656649..2657362	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
ACN002_RS13050	2657575..2658609	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
ACN002_RS13055	2658626..2659504	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
ACN002_RS13060	2659650..2660222	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
ACN002_RS13065	2660222..2660692	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T46519 WP_000911330.1 NZ_CP007491:c2656268-2655870 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T46519 NZ_CP007491:c2656268-2655870 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT46519 WP_000450524.1 NZ_CP007491:c2656495-2656268 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT46519 NZ_CP007491:c2656495-2656268 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33