Detailed information of TA system TA046505

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1365932..1366569
• Replicon: chromosome
• Accession: NZ_CP007472
• Organism: Haemophilus influenzae strain 723

Toxin (Protein)

• Gene name: VapC1
• Uniprot ID: E4QWH2
• Locus tag: NTHI723_RS06790
• Protein ID: WP_005649049.1
• Coordinates: 1365932..1366336 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: VapB1
• Uniprot ID: E4QWH3
• Locus tag: NTHI723_RS06795
• Protein ID: WP_005649046.1
• Coordinates: 1366333..1366569 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NTHI723_RS06760	1361492..1362058	+	567	WP_005544066.1	elongation factor P	-
NTHI723_RS06770	1362409..1362996	-	588	WP_005654063.1	primosomal replication protein	-
NTHI723_RS06775	1363029..1364381	-	1353	WP_005694344.1	Na+/H+ antiporter family protein	-
NTHI723_RS06780	1364695..1365384	-	690	WP_011271988.1	ribonuclease T	-
NTHI723_RS06785	1365458..1365865	-	408	WP_005688980.1	lactoylglutathione lyase	-
NTHI723_RS06790	1365932..1366336	-	405	WP_005649049.1	type II toxin-antitoxin system VapC family toxin	Toxin
NTHI723_RS06795	1366333..1366569	-	237	WP_005649046.1	antitoxin	Antitoxin
NTHI723_RS06800	1366662..1367387	-	726	WP_011271987.1	carboxy-S-adenosyl-L-methionine synthase CmoA	-
NTHI723_RS06805	1367440..1367958	-	519	WP_005649040.1	isoprenylcysteine carboxyl methyltransferase family protein	-
NTHI723_RS06810	1368177..1369943	+	1767	WP_011271986.1	aspartate--tRNA ligase	-
NTHI723_RS06815	1369965..1370438	+	474	WP_011271985.1	dihydroneopterin triphosphate diphosphatase	-
NTHI723_RS06820	1370450..1371190	+	741	WP_005649034.1	YebC/PmpR family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-124)

Antitoxin

• SpoVT-AbrB domain (6-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15692.29 Da
• Isoelectric Point: 8.9777

>T46505 WP_005649049.1 NZ_CP007472:c1366336-1365932 [Haemophilus influenzae]
MIYMLDTNIIIYLMKNRPKIIAERVSQLLPNDRLVMSFITYAELIKGAFGSQNYEQSIRAIELLTERVNVLYPNEQICLH
YGKWANTLKKQGRPIGNNDLWIACHALSLNAVLITHNVKEFQRITDLQWQDWTK

Nucleotide

• Length: 405 bp

>T46505 NZ_CP007472:c1366336-1365932 [Haemophilus influenzae]
ATGATTTATATGTTAGACACCAATATCATTATTTATTTAATGAAAAATCGCCCCAAAATTATTGCCGAACGAGTATCACA
ATTATTGCCTAATGATCGCTTAGTTATGAGCTTTATTACTTATGCTGAACTTATTAAAGGCGCCTTTGGTAGTCAAAATT
ATGAGCAATCAATACGAGCAATAGAATTACTTACTGAACGAGTGAATGTACTATATCCCAATGAACAAATCTGTTTACAT
TATGGCAAATGGGCAAATACACTCAAAAAACAAGGGCGACCTATCGGAAATAATGATCTATGGATCGCTTGTCACGCATT
GAGTTTAAATGCCGTTCTTATTACACATAATGTAAAAGAATTTCAGCGAATTACAGATCTTCAGTGGCAAGATTGGACAA
AATAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9037.19 Da
• Isoelectric Point: 4.2342

>AT46505 WP_005649046.1 NZ_CP007472:c1366569-1366333 [Haemophilus influenzae]
MLTKVFQSGNSQAVRIPMDFRFDVDTVEIFRKENGDVVLRPVSKKTDDFLALFEGFDETFIQALEARDDLPPQERENL

Nucleotide

• Length: 237 bp

>AT46505 NZ_CP007472:c1366569-1366333 [Haemophilus influenzae]
ATGCTTACTAAAGTGTTTCAAAGTGGTAACAGCCAAGCTGTTCGGATCCCGATGGACTTTCGTTTTGACGTCGATACCGT
AGAAATTTTCCGAAAGGAAAATGGGGATGTGGTATTACGCCCAGTTTCTAAAAAAACAGATGATTTTCTTGCGTTATTTG
AAGGATTTGATGAGACCTTTATTCAAGCACTTGAAGCGCGTGATGATTTACCGCCTCAGGAGCGAGAAAATTTATGA

Structures

Toxin

• AlphaFold DB: E4QWH2

Antitoxin

• AlphaFold DB: E4QWH3