46489

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) VapXD/-
Location 1582949..1583427 Replicon chromosome
Accession NZ_CP007470
Organism Haemophilus influenzae strain 477

Toxin (Protein)


Gene name VapD Uniprot ID Q4QN95
Locus tag NTHI477_RS07745 Protein ID WP_011272058.1
Coordinates 1582949..1583227 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name VapX Uniprot ID Q4QN94
Locus tag NTHI477_RS07750 Protein ID WP_011272059.1
Coordinates 1583236..1583427 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NTHI477_RS07725 1578140..1579810 - 1671 WP_044332764.1 fructose-specific PTS transporter subunit EIIC -
NTHI477_RS07730 1579812..1580753 - 942 WP_011272055.1 1-phosphofructokinase -
NTHI477_RS07735 1580755..1582254 - 1500 WP_044332770.1 fused PTS fructose transporter subunit IIA/HPr protein -
NTHI477_RS07740 1582326..1582856 - 531 WP_005649345.1 hypothetical protein -
NTHI477_RS07745 1582949..1583227 - 279 WP_011272058.1 virulence-associated protein VapD Toxin
NTHI477_RS07750 1583236..1583427 - 192 WP_011272059.1 DUF5397 family protein Antitoxin
NTHI477_RS07755 1583499..1584797 - 1299 WP_005691371.1 hemolysin family protein -
NTHI477_RS07760 1584848..1585372 - 525 WP_011272060.1 DUF1523 family protein -
NTHI477_RS07765 1585399..1586181 - 783 WP_011272061.1 YchF/TatD family DNA exonuclease -
NTHI477_RS07770 1586236..1587219 - 984 WP_011272062.1 DNA polymerase III subunit delta' -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Virulence-associated protein D / CRISPR associated protein Cas2

(1-86)

Antitoxin

Protein of unknown function DUF5397

(1-63)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10647.08 Da        Isoelectric Point: 4.9670

>T46489 WP_011272058.1 NZ_CP007470:c1583227-1582949 [Haemophilus influenzae]
MYAIAFDLVVKDTQDYHPKGVQEAYTDIGAVLAKFGFVRTQGSLYTNMNEDMANLFQAMNALKQLAWISQSVRDIRAFRI
EQWSDFTDFIRN

Download         Length: 279 bp

>T46489 NZ_CP007470:c1583227-1582949 [Haemophilus influenzae]
ATGTACGCGATTGCTTTTGATTTAGTTGTAAAAGACACTCAAGATTATCATCCAAAGGGCGTTCAAGAGGCTTATACAGA
CATTGGCGCAGTTTTAGCAAAATTTGGTTTTGTTAGAACACAAGGAAGTTTATATACCAACATGAATGAAGATATGGCGA
ATCTCTTTCAGGCAATGAATGCACTAAAACAGTTGGCGTGGATTTCTCAGTCGGTACGAGATATTCGCGCTTTTCGTATT
GAGCAATGGTCTGATTTTACTGATTTTATACGAAATTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7073.06 Da        Isoelectric Point: 3.8431

>AT46489 WP_011272059.1 NZ_CP007470:c1583427-1583236 [Haemophilus influenzae]
MELRQQIPTGCIKQFGQFGVPYVVGEVAEFLPDGDVLVNITLLQSGEKDIYRLSYLLEDPEAE

Download         Length: 192 bp

>AT46489 NZ_CP007470:c1583427-1583236 [Haemophilus influenzae]
ATGGAACTAAGACAACAAATTCCAACAGGCTGTATTAAGCAATTCGGGCAATTTGGCGTGCCTTATGTGGTTGGCGAGGT
TGCGGAATTTTTGCCAGATGGTGATGTGTTGGTAAATATCACATTGTTACAATCTGGAGAAAAAGACATTTATCGCTTAT
CTTACCTACTTGAAGATCCCGAAGCTGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6ZI0
PDB 6ZN8
PDB 6ZI1

Antitoxin

Source ID Structure
PDB 6ZN8
AlphaFold DB Q4QN94

References