Detailed information of TA system TA046484

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 2332359..2332957
• Replicon: chromosome
• Accession: NZ_CP007456
• Organism: Bifidobacterium kashiwanohense PV20-2

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A0A7I4S3
• Locus tag: AH68_RS09890
• Protein ID: WP_033501891.1
• Coordinates: 2332359..2332670 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: AH68_RS09895
• Protein ID: WP_033501886.1
• Coordinates: 2332664..2332957 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AH68_RS09875	2328479..2329456	-	978	WP_039199571.1	carbohydrate ABC transporter permease	-
AH68_RS09880	2329456..2330367	-	912	WP_039199572.1	sugar ABC transporter permease	-
AH68_RS09885	2330724..2331767	+	1044	WP_039199574.1	LacI family DNA-binding transcriptional regulator	-
AH68_RS09890	2332359..2332670	+	312	WP_033501891.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AH68_RS09895	2332664..2332957	+	294	WP_033501886.1	putative addiction module antidote protein	Antitoxin
AH68_RS10810	2333023..2333211	-	189	WP_158333004.1	hypothetical protein	-
AH68_RS09900	2333264..2335030	-	1767	WP_039199576.1	FAD-dependent oxidoreductase	-
AH68_RS09905	2335134..2335697	-	564	WP_033501889.1	peroxiredoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (11-89)

Antitoxin

• Uncharacterized protein HI1420 (6-89)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11769.66 Da
• Isoelectric Point: 10.5847

>T46484 WP_033501891.1 NZ_CP007456:2332359-2332670 [Bifidobacterium kashiwanohense PV20-2]
MEIQQTDQFRKWLHRLRDHAAKARITARIRQCALAGQPVGDMHPVGKGVNELRFFFGPGYRVYFAQKGNQIMLLLAGGDK
TGQDKTIKEAQKMLESLIKEGQW

Nucleotide

• Length: 312 bp

>T46484 NZ_CP007456:2332359-2332670 [Bifidobacterium kashiwanohense PV20-2]
ATGGAAATCCAACAAACCGACCAGTTCCGCAAGTGGTTGCATAGGCTGCGCGACCATGCCGCCAAAGCGCGGATCACTGC
CCGGATCAGACAGTGTGCACTGGCGGGACAACCGGTCGGCGATATGCACCCGGTCGGTAAAGGCGTGAACGAATTGCGTT
TCTTCTTCGGCCCAGGCTACCGCGTCTACTTCGCCCAGAAAGGCAATCAGATCATGTTGCTGCTCGCCGGCGGGGACAAG
ACCGGACAGGACAAGACCATAAAAGAGGCACAGAAAATGCTCGAATCACTTATCAAGGAGGGACAATGGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10150.46 Da
• Isoelectric Point: 4.3094

>AT46484 WP_033501886.1 NZ_CP007456:2332664-2332957 [Bifidobacterium kashiwanohense PV20-2]
MVSISNFDASEYIENEEDAIAYLNAAAETGDPALLQAAIGDIAKARGMGQIAKEAGVGRESLYKSLNKEGNPSFRTIVKV
VDALGGRLVIEPVPATA

Nucleotide

• Length: 294 bp

>AT46484 NZ_CP007456:2332664-2332957 [Bifidobacterium kashiwanohense PV20-2]
ATGGTAAGCATCAGCAATTTTGATGCCAGCGAATACATCGAAAACGAAGAAGACGCCATCGCATACCTCAACGCCGCCGC
GGAAACCGGCGACCCGGCGCTTCTGCAGGCCGCCATCGGCGACATAGCCAAGGCGCGTGGCATGGGCCAAATCGCCAAGG
AAGCCGGAGTCGGCCGCGAAAGCCTCTACAAAAGCCTCAACAAAGAAGGCAACCCAAGTTTCCGCACCATCGTCAAGGTC
GTCGATGCACTTGGAGGCCGACTTGTCATCGAACCCGTGCCCGCCACGGCATGA

Structures

Toxin

• AlphaFold DB: A0A0A7I4S3