Detailed information of TA system TA046476

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1794607..1795136
• Replicon: chromosome
• Accession: NZ_CP007454
• Organism: Staphylococcus aureus strain 502A isolate RN6607

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CH52_RS09015
• Protein ID: WP_000621175.1
• Coordinates: 1794774..1795136 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: CH52_RS09010
• Protein ID: WP_000948331.1
• Coordinates: 1794607..1794777 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CH52_RS08980	1789644..1790204	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
CH52_RS08985	1790412..1790891	+	480	WP_001287087.1	hypothetical protein	-
CH52_RS08990	1790884..1792467	+	1584	WP_001294626.1	PH domain-containing protein	-
CH52_RS08995	1792454..1792945	+	492	WP_001205912.1	PH domain-containing protein	-
CH52_RS09000	1792949..1793308	+	360	WP_000581197.1	holo-ACP synthase	-
CH52_RS09005	1793374..1794522	+	1149	WP_001281154.1	alanine racemase	-
CH52_RS09010	1794607..1794777	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
CH52_RS09015	1794774..1795136	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CH52_RS09025	1795486..1796487	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
CH52_RS09030	1796606..1796932	+	327	WP_001052491.1	anti-sigma factor antagonist	-
CH52_RS09035	1796934..1797413	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
CH52_RS09040	1797388..1798158	+	771	WP_001041111.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T46476 WP_000621175.1 NZ_CP007454:1794774-1795136 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T46476 NZ_CP007454:1794774-1795136 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT46476 WP_000948331.1 NZ_CP007454:1794607-1794777 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT46476 NZ_CP007454:1794607-1794777 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1