Detailed information of TA system TA046465

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(antitoxin)
• Location: 2501699..2502258
• Replicon: chromosome
• Accession: NZ_CP007451
• Organism: Draconibacterium orientale strain Draconibacterium orientale

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: X5DXE2
• Locus tag: FH5T_RS09910
• Protein ID: WP_038558066.1
• Coordinates: 2502013..2502258 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: X5DFP6
• Locus tag: FH5T_RS09905
• Protein ID: WP_038558063.1
• Coordinates: 2501699..2502022 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FH5T_RS09900	2500410..2501132	+	723	WP_038558062.1	hypothetical protein	-
FH5T_RS09905	2501699..2502022	-	324	WP_038558063.1	type II toxin-antitoxin system PemK/MazF family toxin	Antitoxin
FH5T_RS09910	2502013..2502258	-	246	WP_038558066.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Toxin
FH5T_RS09915	2502754..2504013	+	1260	WP_038558068.1	U32 family peptidase	-
FH5T_RS09920	2504085..2504612	+	528	WP_157470777.1	hypothetical protein	-
FH5T_RS09925	2504653..2504988	+	336	WP_157470780.1	hypothetical protein	-
FH5T_RS09930	2505236..2506144	-	909	WP_038558074.1	sugar phosphate isomerase/epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• mRNA interferase PemK-like (4-103)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9337.82 Da
• Isoelectric Point: 5.1708

>T46465 WP_038558066.1 NZ_CP007451:c2502258-2502013 [Draconibacterium orientale]
MEVSVIKIGNSKGIRLSKTLLDRYNIKDTVDLIMDKGQIIIKPISKPRKGWEKAFEKMAKSGDDNLLIDDVFDDENLEEW
K

Nucleotide

• Length: 246 bp

>T46465 NZ_CP007451:c2502258-2502013 [Draconibacterium orientale]
ATGGAGGTATCAGTTATAAAAATAGGAAATTCAAAGGGGATAAGGTTAAGTAAAACACTGCTTGACAGGTACAACATTAA
AGACACTGTTGATTTAATAATGGATAAAGGGCAGATTATTATAAAACCAATTTCAAAACCGCGCAAAGGATGGGAAAAAG
CTTTTGAAAAAATGGCTAAAAGTGGAGATGACAATTTATTGATTGACGATGTTTTTGACGATGAAAACCTGGAAGAATGG
AAATAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12498.76 Da
• Isoelectric Point: 9.9269

>AT46465 WP_038558063.1 NZ_CP007451:c2502022-2501699 [Draconibacterium orientale]
MEIKQYQIILVNLDPTIGSEIKKTRPCVVISPNEMNKYLRTIVIAPMTSSSKKYPTRVKVKHDNKIGWIVLDQIRTIDRQ
RIIKVFGELQDLEIQELKSVLKETYID

Nucleotide

• Length: 324 bp

>AT46465 NZ_CP007451:c2502022-2501699 [Draconibacterium orientale]
ATGGAAATAAAACAATATCAAATCATTTTAGTCAATCTTGACCCGACAATTGGGAGTGAAATTAAAAAGACCAGACCTTG
TGTGGTAATTTCCCCAAATGAGATGAATAAATATTTGCGGACAATTGTAATTGCGCCAATGACATCAAGTTCAAAAAAAT
ATCCAACACGAGTAAAAGTAAAGCATGACAATAAAATAGGCTGGATTGTTTTAGACCAAATCCGGACAATTGACAGGCAA
AGAATAATAAAAGTTTTTGGAGAATTACAAGATTTAGAAATTCAGGAATTAAAATCGGTTTTAAAAGAAACTTATATTGA
CTAA

Structures

Toxin

• AlphaFold DB: X5DXE2

Antitoxin

• AlphaFold DB: X5DFP6