Detailed information of TA system TA046449

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1277964..1278582
• Replicon: chromosome
• Accession: NZ_CP007448
• Organism: Yersinia enterocolitica LC20

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: Q66DR5
• Locus tag: LC20_RS06130
• Protein ID: WP_002208622.1
• Coordinates: 1277964..1278167 (-)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: LC20_RS06135
• Protein ID: WP_025377437.1
• Coordinates: 1278214..1278582 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LC20_RS06125	1273855..1277046	+	3192	WP_025377436.1	inverse autotransporter beta-barrel domain-containing protein	-
LC20_RS06130	1277964..1278167	-	204	WP_002208622.1	expression modulating protein YmoA	Toxin
LC20_RS06135	1278214..1278582	-	369	WP_025377437.1	Hha toxicity modulator TomB	Antitoxin
LC20_RS06140	1278741..1279094	-	354	WP_025377438.1	hypothetical protein	-
LC20_RS06145	1279867..1280604	+	738	WP_025377439.1	metal ABC transporter ATP-binding protein	-
LC20_RS06150	1280601..1281458	+	858	WP_025377440.1	metal ABC transporter permease	-
LC20_RS06155	1281487..1282365	+	879	WP_087769094.1	metal ABC transporter substrate-binding protein	-
LC20_RS06160	1282483..1282626	-	144	WP_002208618.1	type B 50S ribosomal protein L36	-
LC20_RS06165	1282642..1282902	-	261	WP_025377443.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-118)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8063.31 Da
• Isoelectric Point: 6.4573

>T46449 WP_002208622.1 NZ_CP007448:c1278167-1277964 [Yersinia enterocolitica LC20]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPPTVWQHVK

Nucleotide

• Length: 204 bp

>T46449 NZ_CP007448:c1278167-1277964 [Yersinia enterocolitica LC20]
ATGACAAAAACTGACTACCTGATGCGTTTAAGAAAATGTACGACTATTGATACGTTGGAGCGTGTAATTGAAAAAAATAA
GTATGAACTTTCAGATGATGAGTTGGAATTGTTTTACTCAGCAGCAGACCATCGCTTAGCTGAACTCACTATGAATAAAC
TTTATGATAAAATTCCACCTACTGTTTGGCAACATGTGAAGTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14234.98 Da
• Isoelectric Point: 4.4314

>AT46449 WP_025377437.1 NZ_CP007448:c1278582-1278214 [Yersinia enterocolitica LC20]
MDEYSPKRHDIAQLKFLCESLYDEGIATLGDSHHGWVNDPTSAVNLQLNDLIEHIASFVMSFKIKYPDDGDLSELVEEYL
DDTYTLFSSYGINDPELRRWQKTKERLFRLFSGEYICTLMKT

Nucleotide

• Length: 369 bp

>AT46449 NZ_CP007448:c1278582-1278214 [Yersinia enterocolitica LC20]
ATGGATGAGTACTCACCTAAGCGGCATGACATTGCCCAATTGAAGTTCCTATGTGAGAGTTTGTATGATGAAGGCATTGC
TACCCTTGGTGATAGTCACCACGGCTGGGTAAATGACCCAACTTCTGCCGTAAACTTACAACTTAATGATTTGATCGAGC
ATATAGCTTCATTTGTGATGAGTTTTAAAATAAAATACCCTGATGACGGTGACCTTTCGGAGTTAGTGGAAGAATATTTA
GACGATACATATACTCTGTTTAGTAGCTACGGAATCAATGATCCTGAATTGCGGCGCTGGCAAAAAACCAAAGAGCGTTT
ATTCAGACTTTTCTCAGGGGAATACATCTGTACCCTGATGAAAACTTAA

Structures

Toxin

• PDB: 2K5S