Detailed information of TA system TA046446

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 556656..557295
• Replicon: chromosome
• Accession: NZ_CP007446
• Organism: Snodgrassella alvi wkB2

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: X2HE68
• Locus tag: SALWKB2_RS02515
• Protein ID: WP_025330119.1
• Coordinates: 557113..557295 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: X2H602
• Locus tag: SALWKB2_RS02510
• Protein ID: WP_025330118.1
• Coordinates: 556656..557081 (-)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SALWKB2_RS02455	551826..552392	+	567	WP_099048780.1	hypothetical protein	-
SALWKB2_RS02460	552407..552664	+	258	WP_144353315.1	hypothetical protein	-
SALWKB2_RS02465	552661..553176	+	516	WP_025330109.1	hypothetical protein	-
SALWKB2_RS02470	553166..553519	+	354	WP_025330110.1	hypothetical protein	-
SALWKB2_RS02475	553449..553796	+	348	WP_025330111.1	hypothetical protein	-
SALWKB2_RS02480	553799..554398	+	600	WP_025330112.1	DUF1367 family protein	-
SALWKB2_RS02485	554401..554694	+	294	WP_025330113.1	DUF1364 domain-containing protein	-
SALWKB2_RS02490	554705..555205	+	501	WP_038648687.1	hypothetical protein	-
SALWKB2_RS02495	555368..555799	-	432	WP_025330115.1	type II toxin-antitoxin system HicB family antitoxin	-
SALWKB2_RS02500	555961..556134	-	174	WP_025330116.1	type II toxin-antitoxin system HicA family toxin	-
SALWKB2_RS02505	556411..556659	-	249	WP_025330117.1	helix-turn-helix domain-containing protein	-
SALWKB2_RS02510	556656..557081	-	426	WP_025330118.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
SALWKB2_RS02515	557113..557295	-	183	WP_025330119.1	type II toxin-antitoxin system HicA family toxin	Toxin
SALWKB2_RS02520	557481..558029	+	549	WP_198209771.1	terminase small subunit	-
SALWKB2_RS02525	558022..559365	+	1344	WP_025330121.1	PBSX family phage terminase large subunit	-
SALWKB2_RS02530	559362..560915	+	1554	WP_025330122.1	DUF1073 domain-containing protein	-
SALWKB2_RS02535	560866..561669	+	804	WP_025330123.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	532252..585782	53530

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (5-126)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6827.97 Da
• Isoelectric Point: 10.9086

>T46446 WP_025330119.1 NZ_CP007446:c557295-557113 [Snodgrassella alvi wkB2]
MHSSKVIKRLLDDGWYEVAVRGSHHQFKHPEKSGRVTVPHPKKDLPLGTVKNIFKQAGLK

Nucleotide

• Length: 183 bp

>T46446 NZ_CP007446:c557295-557113 [Snodgrassella alvi wkB2]
ATGCATAGCTCAAAGGTTATTAAAAGGCTGCTTGATGATGGGTGGTATGAGGTAGCCGTTAGAGGCAGCCATCATCAATT
CAAACATCCGGAAAAATCAGGTCGTGTTACCGTCCCTCACCCTAAAAAGGATTTACCACTCGGGACAGTAAAAAATATAT
TTAAACAAGCCGGTCTTAAATAA

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15742.73 Da
• Isoelectric Point: 4.5106

>AT46446 WP_025330118.1 NZ_CP007446:c557081-556656 [Snodgrassella alvi wkB2]
MFLYIAIHKDENTGYGVTVPSLPGCFSYGDTLEKAIEESKQAILFHIEGLLEDGIEPETNQPDLATLIDTPEYAGAQWFG
IEVNIDHLTLKPERFNVSWPKYLLNKVDTYVSLTHDTRSNFLAKAALERINSANQNAGIAK

Nucleotide

• Length: 426 bp

>AT46446 NZ_CP007446:c557081-556656 [Snodgrassella alvi wkB2]
ATGTTTCTCTACATTGCTATACACAAAGATGAAAACACCGGTTACGGTGTAACAGTGCCCTCTTTACCCGGCTGCTTTTC
ATATGGTGATACTTTAGAAAAGGCTATTGAAGAATCTAAACAAGCCATCTTATTTCATATCGAGGGTTTACTTGAAGATG
GCATAGAACCTGAAACAAATCAGCCCGACCTTGCCACTCTCATTGATACCCCTGAGTATGCAGGAGCACAATGGTTTGGT
ATCGAGGTGAATATTGATCACCTAACCCTTAAACCAGAACGATTTAATGTTAGCTGGCCTAAATACCTTTTGAACAAGGT
TGATACCTACGTATCACTAACCCACGATACCCGTTCTAACTTTCTTGCTAAAGCCGCACTTGAGAGAATTAATAGTGCCA
ATCAAAACGCCGGCATAGCTAAATGA

Structures

Toxin

• AlphaFold DB: X2HE68

Antitoxin

• AlphaFold DB: X2H602