Detailed information of TA system TA046445

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4782656..4783476
• Replicon: chromosome
• Accession: NZ_CP007442
• Organism: Escherichia coli ACN001

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: B6I030
• Locus tag: J444_RS23575
• Protein ID: WP_001054379.1
• Coordinates: 4783219..4783476 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: J444_RS23570
• Protein ID: WP_000123957.1
• Coordinates: 4782656..4783207 (-)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J444_RS23545	4778350..4779663	-	1314	WP_000460845.1	galactitol-specific PTS transporter subunit IIC	-
J444_RS23550	4779675..4779953	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
J444_RS23555	4779950..4781071	-	1122	WP_000010833.1	M42 family metallopeptidase	-
J444_RS27555	4781316..4781432	-	117	Protein_4464	VOC family protein	-
J444_RS23560	4781470..4781730	-	261	WP_000077646.1	hypothetical protein	-
J444_RS23565	4781858..4782604	-	747	WP_001588380.1	class I SAM-dependent methyltransferase	-
J444_RS23570	4782656..4783207	-	552	WP_000123957.1	N-acetyltransferase	Antitoxin
J444_RS23575	4783219..4783476	-	258	WP_001054379.1	YjhX family toxin	Toxin
J444_RS23580	4783853..4784152	-	300	WP_001332034.1	GNAT family N-acetyltransferase	-
J444_RS23585	4784230..4785456	+	1227	Protein_4470	helicase YjhR	-
J444_RS23590	4786039..4787019	-	981	WP_000991462.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
J444_RS23595	4787083..4788189	-	1107	WP_001295733.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (19-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9568.07 Da
• Isoelectric Point: 11.1381

>T46445 WP_001054379.1 NZ_CP007442:c4783476-4783219 [Escherichia coli ACN001]
MNLSRQEQRTLHVLAKGGRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTGLNNVRA
QLDNR

Nucleotide

• Length: 258 bp

>T46445 NZ_CP007442:c4783476-4783219 [Escherichia coli ACN001]
ATGAATTTATCCCGTCAGGAACAACGTACGTTACACGTTCTCGCTAAAGGTGGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGTCAGCCCTATCGAATCAACACTACCGGGCTGAATAACGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20358.34 Da
• Isoelectric Point: 6.4182

>AT46445 WP_000123957.1 NZ_CP007442:c4783207-4782656 [Escherichia coli ACN001]
MTAHHFTFQITDESDASDIREVETRAFGFSKEADLTASLLEDESARPALSLLARHEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQALTA
QPMNVTGHIQCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT46445 NZ_CP007442:c4783207-4782656 [Escherichia coli ACN001]
ATGACAGCACACCACTTCACTTTTCAAATCACAGATGAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAACGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCTGCGTTATCGCTGCTGGCCC
GACATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGGGAAATGGATTCACCGCTGATGCAT
ATCCTCGCTCCCCTGGCAGTCATACCGGAATACCAGGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAAGCACTCACCGCT
CAGCCCATGAACGTGACCGGCCACATCCAGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA

Structures

Toxin

• AlphaFold DB: B6I030