Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4675095..4675690 Replicon chromosome
Accession NZ_CP007442
Organism Escherichia coli ACN001

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag J444_RS23020 Protein ID WP_000239579.1
Coordinates 4675340..4675690 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag J444_RS23015 Protein ID WP_001223208.1
Coordinates 4675095..4675346 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J444_RS23005 4670759..4674538 + 3780 WP_048943320.1 autotransporter assembly complex protein TamB -
J444_RS23010 4674541..4674882 + 342 WP_001219160.1 gamma-glutamylcyclotransferase -
J444_RS23015 4675095..4675346 + 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
J444_RS23020 4675340..4675690 + 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
J444_RS23025 4675770..4676300 - 531 WP_000055072.1 inorganic diphosphatase -
J444_RS23030 4676610..4677566 + 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
J444_RS23035 4677876..4679378 + 1503 WP_000205793.1 sugar ABC transporter ATP-binding protein -
J444_RS23040 4679392..4680414 + 1023 WP_001339490.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T46444 WP_000239579.1 NZ_CP007442:4675340-4675690 [Escherichia coli ACN001]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T46444 NZ_CP007442:4675340-4675690 [Escherichia coli ACN001]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT46444 WP_001223208.1 NZ_CP007442:4675095-4675346 [Escherichia coli ACN001]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT46444 NZ_CP007442:4675095-4675346 [Escherichia coli ACN001]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References