Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3124715..3125369 Replicon chromosome
Accession NZ_CP007442
Organism Escherichia coli ACN001

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag J444_RS15345 Protein ID WP_000244777.1
Coordinates 3124715..3125122 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag J444_RS15350 Protein ID WP_000354046.1
Coordinates 3125103..3125369 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J444_RS15325 3120672..3122405 - 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -
J444_RS15330 3122411..3123121 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
J444_RS15335 3123146..3124042 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
J444_RS15340 3124154..3124675 + 522 WP_001055874.1 flavodoxin FldB -
J444_RS15345 3124715..3125122 - 408 WP_000244777.1 toxin CptA Toxin
J444_RS15350 3125103..3125369 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
J444_RS15355 3125612..3126592 + 981 WP_000886061.1 tRNA-modifying protein YgfZ -
J444_RS15360 3126669..3127328 - 660 WP_000250274.1 hemolysin III family protein -
J444_RS15365 3127492..3127803 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
J444_RS15370 3127848..3129281 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
J444_RS15375 3129338..3130081 - 744 WP_000951948.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T46438 WP_000244777.1 NZ_CP007442:c3125122-3124715 [Escherichia coli ACN001]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T46438 NZ_CP007442:c3125122-3124715 [Escherichia coli ACN001]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT46438 WP_000354046.1 NZ_CP007442:c3125369-3125103 [Escherichia coli ACN001]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT46438 NZ_CP007442:c3125369-3125103 [Escherichia coli ACN001]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References