Detailed information of TA system TA046435

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2648034..2648659
• Replicon: chromosome
• Accession: NZ_CP007442
• Organism: Escherichia coli ACN001

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: J444_RS12940
• Protein ID: WP_000911330.1
• Coordinates: 2648034..2648432 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: J444_RS12945
• Protein ID: WP_000450524.1
• Coordinates: 2648432..2648659 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J444_RS12920	2643912..2644112	+	201	WP_000383836.1	YpfN family protein	-
J444_RS12925	2644222..2644920	-	699	WP_000679823.1	esterase	-
J444_RS12930	2644994..2647009	-	2016	WP_000829323.1	tRNA cytosine(34) acetyltransferase TmcA	-
J444_RS12935	2647024..2647887	-	864	WP_001267507.1	neutral zinc metallopeptidase	-
J444_RS12940	2648034..2648432	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
J444_RS12945	2648432..2648659	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
J444_RS12950	2648813..2649526	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
J444_RS12960	2649739..2650773	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
J444_RS12965	2650790..2651668	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
J444_RS12970	2651814..2652386	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
J444_RS12975	2652386..2652856	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T46435 WP_000911330.1 NZ_CP007442:c2648432-2648034 [Escherichia coli ACN001]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T46435 NZ_CP007442:c2648432-2648034 [Escherichia coli ACN001]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT46435 WP_000450524.1 NZ_CP007442:c2648659-2648432 [Escherichia coli ACN001]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT46435 NZ_CP007442:c2648659-2648432 [Escherichia coli ACN001]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33