46424

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 448344..448962 Replicon chromosome
Accession NZ_CP007442
Organism Escherichia coli ACN001

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag J444_RS02140 Protein ID WP_001291435.1
Coordinates 448344..448562 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag J444_RS02145 Protein ID WP_000344800.1
Coordinates 448588..448962 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J444_RS02110 443633..444205 + 573 WP_000779831.1 YbaY family lipoprotein -
J444_RS02115 444236..444547 - 312 WP_000409911.1 MGMT family protein -
J444_RS02120 444926..445279 + 354 Protein_401 DUF1428 family protein -
J444_RS02125 445321..446871 - 1551 WP_048943370.1 cyclic-guanylate-specific phosphodiesterase PdeB -
J444_RS02130 447035..447505 - 471 WP_000136192.1 YlaC family protein -
J444_RS02135 447621..448172 - 552 WP_000102568.1 maltose O-acetyltransferase -
J444_RS02140 448344..448562 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
J444_RS02145 448588..448962 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
J444_RS02150 449508..452657 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
J444_RS02155 452680..453873 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T46424 WP_001291435.1 NZ_CP007442:c448562-448344 [Escherichia coli ACN001]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T46424 NZ_CP007442:c448562-448344 [Escherichia coli ACN001]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT46424 WP_000344800.1 NZ_CP007442:c448962-448588 [Escherichia coli ACN001]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT46424 NZ_CP007442:c448962-448588 [Escherichia coli ACN001]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References