Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 529951..530587 Replicon chromosome
Accession NZ_CP007436
Organism Bacillus pumilus strain B6033

Toxin (Protein)


Gene name mazF Uniprot ID K2MHT4
Locus tag BW16_RS02545 Protein ID WP_003214169.1
Coordinates 530237..530587 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID W8QJ31
Locus tag BW16_RS02540 Protein ID WP_003214273.1
Coordinates 529951..530232 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BW16_RS02520 526105..526710 - 606 WP_007496491.1 rhomboid family intramembrane serine protease -
BW16_RS02525 526805..527170 + 366 WP_017366663.1 holo-ACP synthase -
BW16_RS02530 527331..528347 + 1017 WP_123796446.1 outer membrane lipoprotein carrier protein LolA -
BW16_RS02535 528476..529657 + 1182 WP_025206795.1 alanine racemase -
BW16_RS02540 529951..530232 + 282 WP_003214273.1 hypothetical protein Antitoxin
BW16_RS02545 530237..530587 + 351 WP_003214169.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BW16_RS02550 530702..531532 + 831 WP_007496484.1 STAS domain-containing protein -
BW16_RS02555 531537..531905 + 369 WP_003214235.1 RsbT antagonist protein RsbS -
BW16_RS02560 531908..532309 + 402 WP_003214085.1 anti-sigma regulatory factor -
BW16_RS02565 532320..533327 + 1008 WP_008346904.1 PP2C family protein-serine/threonine phosphatase -
BW16_RS02570 533387..533716 + 330 WP_008346902.1 anti-sigma factor antagonist -
BW16_RS02575 533713..534201 + 489 WP_007496471.1 anti-sigma B factor RsbW -
BW16_RS02580 534167..534955 + 789 WP_025206796.1 RNA polymerase sigma factor SigB -
BW16_RS02585 534955..535554 + 600 WP_008346897.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12976.99 Da        Isoelectric Point: 5.1663

>T46419 WP_003214169.1 NZ_CP007436:530237-530587 [Bacillus pumilus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEINAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQVSLALIDF

Download         Length: 351 bp

>T46419 NZ_CP007436:530237-530587 [Bacillus pumilus]
TTGATTGTTAAACGCGGTGATGTTTACTTTGCTGATTTATCTCCTGTTGTTGGCTCCGAGCAGGGCGGGGTTCGCCCGGT
ATTGGTGATTCAAAATGACATTGGAAATCGCTTCAGCCCGACTGCTATCGTTGCAGCCATAACAGCCCAAATACAAAAAG
CGAAATTACCGACTCACGTCGAAATTAATGCAAAGCGCTACGGCTTTGAACGAGATTCTGTCATTCTTTTGGAACAGATT
CGTACGATCGATAAACAAAGATTGACAGATAAAATCACACATCTTGATGATGAGATGATGGAGAAGGTTGATGAAGCCTT
ACAAGTTAGTTTGGCTCTTATTGATTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10864.25 Da        Isoelectric Point: 5.2726

>AT46419 WP_003214273.1 NZ_CP007436:529951-530232 [Bacillus pumilus]
MSESSATNEMKIRLPEALMSEIERVAEQDKVTRNELIYRATKTYIRERNIRQNRESMRRGYMEMAKINLNISSEAQYAEF
EAENTVERLVSGG

Download         Length: 282 bp

>AT46419 NZ_CP007436:529951-530232 [Bacillus pumilus]
TTGTCTGAATCCAGCGCAACAAACGAAATGAAGATTCGACTGCCGGAGGCTTTAATGTCTGAGATCGAACGAGTAGCAGA
GCAGGATAAGGTAACAAGAAATGAATTGATCTACCGAGCGACAAAAACGTATATTCGTGAACGTAATATTCGACAAAATC
GTGAATCGATGAGACGCGGCTACATGGAGATGGCGAAAATTAATCTGAATATCTCTTCAGAGGCACAATACGCAGAATTT
GAGGCTGAGAATACAGTTGAGCGATTAGTCAGCGGGGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K2MHT4

Antitoxin

Source ID Structure
AlphaFold DB A0A081L854

References