Detailed information of TA system TA046415

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 2257272..2257876
• Replicon: chromosome
• Accession: NZ_CP007410
• Organism: Pseudomonas brassicacearum strain DF41

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: W8PHH8
• Locus tag: CD58_RS09840
• Protein ID: WP_025212842.1
• Coordinates: 2257583..2257876 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: W8PQG3
• Locus tag: CD58_RS09835
• Protein ID: WP_025212841.1
• Coordinates: 2257272..2257580 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CD58_RS09800	2253213..2253830	+	618	WP_025212834.1	hypothetical protein	-
CD58_RS09805	2253948..2254589	+	642	WP_025212835.1	hypothetical protein	-
CD58_RS09810	2254714..2255250	-	537	WP_025212836.1	DUF2867 domain-containing protein	-
CD58_RS09815	2255283..2255930	-	648	WP_025212837.1	DJ-1/PfpI family protein	-
CD58_RS29155	2256057..2256611	+	555	WP_080712529.1	TetR/AcrR family transcriptional regulator	-
CD58_RS09825	2256699..2256902	+	204	WP_025212839.1	hypothetical protein	-
CD58_RS09830	2256935..2257219	+	285	WP_025212840.1	hypothetical protein	-
CD58_RS09835	2257272..2257580	-	309	WP_025212841.1	putative addiction module antidote protein	Antitoxin
CD58_RS09840	2257583..2257876	-	294	WP_025212842.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CD58_RS09845	2258217..2258489	+	273	WP_025212843.1	hypothetical protein	-
CD58_RS09850	2259044..2259928	-	885	WP_025212844.1	LysR family transcriptional regulator	-
CD58_RS09855	2260035..2260484	+	450	WP_025212845.1	PACE efflux transporter	-
CD58_RS29165	2260531..2260707	-	177	WP_080712531.1	DUF3606 domain-containing protein	-
CD58_RS09860	2260900..2261100	+	201	WP_025212846.1	hypothetical protein	-
CD58_RS09865	2261255..2261524	+	270	WP_025212847.1	YceK/YidQ family lipoprotein	-
CD58_RS09870	2261656..2262084	-	429	WP_025212848.1	DUF2628 domain-containing protein	-
CD58_RS09875	2262349..2262777	-	429	WP_025212849.1	HD domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2251236..2264669	13433

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (30-91)

Antitoxin

• Uncharacterized protein HI1420 (5-93)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10560.19 Da
• Isoelectric Point: 10.8628

>T46415 WP_025212842.1 NZ_CP007410:c2257876-2257583 [Pseudomonas brassicacearum]
MNYLIQQTQTFTAWHSSVRDLRAKIAIARRIDRAAAGNLGDVKPVGDGISELRVDVGAGYRVYFTMRGGVVIVLLAGGDK
SSQSADIRRAKKLAKEI

Nucleotide

• Length: 294 bp

>T46415 NZ_CP007410:c2257876-2257583 [Pseudomonas brassicacearum]
ATGAATTATCTCATCCAGCAAACGCAGACGTTCACTGCCTGGCATTCATCGGTTCGTGATCTGCGAGCAAAAATCGCTAT
CGCCCGCCGCATTGATCGCGCCGCAGCAGGTAACCTGGGCGATGTGAAACCTGTCGGTGATGGTATTTCAGAATTGCGTG
TGGATGTAGGCGCTGGCTACCGGGTCTATTTCACCATGCGCGGTGGAGTGGTCATCGTGCTATTGGCTGGTGGTGACAAG
TCTTCACAGAGTGCTGATATCCGGCGAGCGAAGAAATTGGCAAAGGAGATTTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 10958.37 Da
• Isoelectric Point: 5.9574

>AT46415 WP_025212841.1 NZ_CP007410:c2257580-2257272 [Pseudomonas brassicacearum]
MSQHLTTFDMAALLDSDEAISEYLSQVLADGDSEEFLRAIGYVAKARGMAQIAKDSGMGRESLYKAFAPGSKPRFDTVLK
VIHALGIDLHAQPGHATNHSPA

Nucleotide

• Length: 309 bp

>AT46415 NZ_CP007410:c2257580-2257272 [Pseudomonas brassicacearum]
ATGAGCCAACACTTGACGACATTCGACATGGCGGCACTACTCGATAGCGACGAAGCGATCAGTGAATACCTTTCTCAGGT
GTTGGCTGATGGTGATAGCGAGGAGTTTCTCCGAGCGATCGGCTATGTGGCCAAGGCCCGAGGCATGGCGCAGATCGCTA
AAGACTCCGGCATGGGACGAGAAAGCTTGTACAAGGCGTTCGCCCCCGGATCAAAACCGAGATTCGATACCGTACTCAAG
GTCATTCATGCGCTAGGCATTGATCTTCATGCGCAGCCTGGGCATGCGACTAATCATTCACCTGCCTGA