46412

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 506355..506991 Replicon chromosome
Accession NZ_CP007409
Organism Bacillus subtilis subsp. subtilis str. OH 131.1 strain OH131.1

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag Q433_RS02585 Protein ID WP_003156187.1
Coordinates 506641..506991 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag Q433_RS02580 Protein ID WP_003225183.1
Coordinates 506355..506636 (+) Length 94 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Q433_RS02560 502715..503314 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
Q433_RS02565 503409..503774 + 366 WP_015252768.1 holo-ACP synthase -
Q433_RS02570 503940..504956 + 1017 WP_072557167.1 outer membrane lipoprotein carrier protein LolA -
Q433_RS02575 505070..506239 + 1170 WP_029727188.1 alanine racemase -
Q433_RS02580 506355..506636 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
Q433_RS02585 506641..506991 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
Q433_RS02590 507107..507931 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
Q433_RS02595 507936..508301 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
Q433_RS02600 508305..508706 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
Q433_RS02605 508718..509725 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
Q433_RS02610 509787..510116 + 330 WP_014475877.1 anti-sigma factor antagonist RsbV -
Q433_RS02615 510113..510595 + 483 WP_038427419.1 anti-sigma B factor RsbW -
Q433_RS02620 510561..511349 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
Q433_RS02625 511349..511948 + 600 WP_003234303.1 phosphoserine phosphatase RsbX -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-111)

Antitoxin

(12-45)


Sequences


Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T46412 WP_003156187.1 NZ_CP007409:506641-506991 [Bacillus subtilis subsp. subtilis str. OH 131.1]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T46412 NZ_CP007409:506641-506991 [Bacillus subtilis subsp. subtilis str. OH 131.1]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT46412 WP_003225183.1 NZ_CP007409:506355-506636 [Bacillus subtilis subsp. subtilis str. OH 131.1]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT46412 NZ_CP007409:506355-506636 [Bacillus subtilis subsp. subtilis str. OH 131.1]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8


Antitoxin

Source ID Structure
AlphaFold DB G4NU32

References