Detailed information of TA system TA046412

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 506355..506991
• Replicon: chromosome
• Accession: NZ_CP007409
• Organism: Bacillus subtilis subsp. subtilis str. OH 131.1 strain OH131.1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: Q433_RS02585
• Protein ID: WP_003156187.1
• Coordinates: 506641..506991 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: Q433_RS02580
• Protein ID: WP_003225183.1
• Coordinates: 506355..506636 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Q433_RS02560	502715..503314	-	600	WP_003246687.1	rhomboid family intramembrane serine protease	-
Q433_RS02565	503409..503774	+	366	WP_015252768.1	holo-ACP synthase	-
Q433_RS02570	503940..504956	+	1017	WP_072557167.1	outer membrane lipoprotein carrier protein LolA	-
Q433_RS02575	505070..506239	+	1170	WP_029727188.1	alanine racemase	-
Q433_RS02580	506355..506636	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
Q433_RS02585	506641..506991	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
Q433_RS02590	507107..507931	+	825	WP_009966610.1	RsbT co-antagonist protein RsbRA	-
Q433_RS02595	507936..508301	+	366	WP_003225190.1	RsbT antagonist protein RsbS	-
Q433_RS02600	508305..508706	+	402	WP_003246640.1	serine/threonine-protein kinase RsbT	-
Q433_RS02605	508718..509725	+	1008	WP_003234295.1	phosphoserine phosphatase RsbU	-
Q433_RS02610	509787..510116	+	330	WP_014475877.1	anti-sigma factor antagonist RsbV	-
Q433_RS02615	510113..510595	+	483	WP_038427419.1	anti-sigma B factor RsbW	-
Q433_RS02620	510561..511349	+	789	WP_003246715.1	RNA polymerase sigma factor SigB	-
Q433_RS02625	511349..511948	+	600	WP_003234303.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T46412 WP_003156187.1 NZ_CP007409:506641-506991 [Bacillus subtilis subsp. subtilis str. OH 131.1]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T46412 NZ_CP007409:506641-506991 [Bacillus subtilis subsp. subtilis str. OH 131.1]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT46412 WP_003225183.1 NZ_CP007409:506355-506636 [Bacillus subtilis subsp. subtilis str. OH 131.1]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT46412 NZ_CP007409:506355-506636 [Bacillus subtilis subsp. subtilis str. OH 131.1]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• PDB: 4ME7
• AlphaFold DB: G4NU32