Detailed information of TA system TA046405

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 4952732..4953273
• Replicon: chromosome
• Accession: NZ_CP007394
• Organism: Escherichia coli strain ST2747

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: CF60_RS24060
• Protein ID: WP_025263483.1
• Coordinates: 4952732..4953025 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: T1PXV2
• Locus tag: CF60_RS24065
• Protein ID: WP_001139206.1
• Coordinates: 4953022..4953273 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CF60_RS24045	4948374..4951871	+	3498	WP_025263481.1	autotransporter outer membrane beta-barrel domain-containing protein	-
CF60_RS27510	4952046..4952251	-	206	Protein_4533	transposase	-
CF60_RS24055	4952241..4952444	+	204	WP_038429594.1	alkaline phosphatase	-
CF60_RS24060	4952732..4953025	-	294	WP_025263483.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CF60_RS24065	4953022..4953273	-	252	WP_001139206.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CF60_RS24075	4954220..4955092	-	873	WP_000729436.1	incFII family plasmid replication initiator RepA	-
CF60_RS28100	4955253..4955708	+	456	WP_025263484.1	hypothetical protein	-
CF60_RS24080	4955956..4956492	+	537	WP_001217006.1	transcription termination factor NusG	-
CF60_RS28280	4956525..4956695	-	171	WP_157918115.1	hypothetical protein	-
CF60_RS24085	4957040..4957324	+	285	WP_025263485.1	hypothetical protein	-
CF60_RS24090	4957907..4958251	+	345	WP_001026850.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11428.46 Da
• Isoelectric Point: 10.5077

>T46405 WP_025263483.1 NZ_CP007394:c4953025-4952732 [Escherichia coli]
MTYTVKFRDDALKEWLRLDKTIQQQFAKKLKKCSENPHIASAQLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERERNNVYHLASERMK

Nucleotide

• Length: 294 bp

>T46405 NZ_CP007394:c4953025-4952732 [Escherichia coli]
ATGACTTATACAGTAAAATTCCGGGATGATGCACTGAAAGAATGGCTCAGACTGGACAAGACCATTCAGCAGCAGTTCGC
AAAAAAACTGAAAAAATGCAGTGAAAATCCTCATATAGCTTCTGCACAACTACGTGGATTAAAGGACTGCTATAAAATCA
AACTTCGCGCATCAGGTTTTCGTCTGGTTTATCAGGTTATTGATGATATGTTAATTATTGCGGTCGTCGCAGTAGGTAAA
CGTGAACGTGAACGCAACAACGTTTATCATCTCGCCAGTGAACGTATGAAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9171.39 Da
• Isoelectric Point: 4.0927

>AT46405 WP_001139206.1 NZ_CP007394:c4953273-4953022 [Escherichia coli]
MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSSQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT46405 NZ_CP007394:c4953273-4953022 [Escherichia coli]
ATGCCCAGTATCATTTTGTCTGACACCAGTGCAAGCGTCAGTGAGCTGAAAAAAAATCCGATGGCAACTGTCAGTGCCGG
AGAGGGGTATCCTGTTGCCATTCTTAACCGGAATCAGCCAGCATTCTACTGTGTCCCTGCTGAGCTCTATGAAAGAATGC
TTGATGCCCTGGATGACCAGGAGCTGGTAAAACTGGTTACTGAACGCAGCAGTCAGCCTCTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Structures

Antitoxin

• AlphaFold DB: T1PXV2