Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3824635..3825289 Replicon chromosome
Accession NZ_CP007394
Organism Escherichia coli strain ST2747

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag CF60_RS18465 Protein ID WP_000244781.1
Coordinates 3824635..3825042 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag CF60_RS18470 Protein ID WP_000354046.1
Coordinates 3825023..3825289 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CF60_RS18445 3820592..3822325 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
CF60_RS18450 3822331..3823041 - 711 WP_025210838.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CF60_RS18455 3823066..3823962 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
CF60_RS18460 3824074..3824595 + 522 WP_001055874.1 flavodoxin FldB -
CF60_RS18465 3824635..3825042 - 408 WP_000244781.1 protein YgfX Toxin
CF60_RS18470 3825023..3825289 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
CF60_RS18475 3825532..3826512 + 981 WP_001587401.1 tRNA-modifying protein YgfZ -
CF60_RS18480 3826589..3827248 - 660 WP_000250274.1 hemolysin III family protein -
CF60_RS18485 3827412..3827723 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
CF60_RS18490 3827768..3829201 + 1434 WP_025210839.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T46401 WP_000244781.1 NZ_CP007394:c3825042-3824635 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T46401 NZ_CP007394:c3825042-3824635 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATCTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATCCACGGGTTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT46401 WP_000354046.1 NZ_CP007394:c3825289-3825023 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT46401 NZ_CP007394:c3825289-3825023 [Escherichia coli]
ATGGATATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAACCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References