Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 450659..451181 Replicon chromosome
Accession NZ_CP007394
Organism Escherichia coli strain ST2747

Toxin (Protein)


Gene name relE Uniprot ID A0A829L6G8
Locus tag CF60_RS02160 Protein ID WP_001105433.1
Coordinates 450891..451181 (+) Length 97 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0D8WJ36
Locus tag CF60_RS02155 Protein ID WP_000212718.1
Coordinates 450659..450901 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CF60_RS02135 446572..447945 + 1374 WP_001219792.1 UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase -
CF60_RS02140 448028..448579 - 552 WP_000166270.1 ribosome-associated protein -
CF60_RS02145 448673..450025 + 1353 WP_001162171.1 metalloprotease PmbA -
CF60_RS02150 450081..450467 + 387 WP_001232253.1 cytochrome b562 -
CF60_RS02155 450659..450901 + 243 WP_000212718.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
CF60_RS02160 450891..451181 + 291 WP_001105433.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CF60_RS02165 451182..451646 - 465 WP_001009182.1 anaerobic ribonucleoside-triphosphate reductase-activating protein -
CF60_RS02175 451826..453964 - 2139 WP_000187798.1 anaerobic ribonucleoside-triphosphate reductase -
CF60_RS02180 454358..456013 - 1656 WP_001577606.1 alpha,alpha-phosphotrehalase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-66)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11367.23 Da        Isoelectric Point: 10.0238

>T46379 WP_001105433.1 NZ_CP007394:450891-451181 [Escherichia coli]
MNYELAFDPRALKEWQKLGVTVREQFKKKLEDVLKRPRNPSAKLRDLPDCYKIKLRTQGYRLVYQVNDKELLVLVIAIGK
RENSAVYEDATKRLDE

Download         Length: 291 bp

>T46379 NZ_CP007394:450891-451181 [Escherichia coli]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACAGGGATATCGCCTGGTTTATCAGGTTAACGATAAAGAGTTGCTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACTAAACGGCTGGATGAGTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9174.63 Da        Isoelectric Point: 5.1369

>AT46379 WP_000212718.1 NZ_CP007394:450659-450901 [Escherichia coli]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAENGRMPVKTLTVSDSEEALLRTVRERLAYPQEAIKVRLDEL

Download         Length: 243 bp

>AT46379 NZ_CP007394:450659-450901 [Escherichia coli]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATCACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCTACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829L6G8

Antitoxin

Source ID Structure
AlphaFold DB A0A0D8WJ36

References