Detailed information of TA system TA046378

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 439242..439837
• Replicon: chromosome
• Accession: NZ_CP007394
• Organism: Escherichia coli strain ST2747

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: CF60_RS02100
• Protein ID: WP_000239581.1
• Coordinates: 439487..439837 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: V0YZQ7
• Locus tag: CF60_RS02095
• Protein ID: WP_001223206.1
• Coordinates: 439242..439493 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CF60_RS02085	434907..438686	+	3780	WP_001577602.1	autotransporter assembly complex protein TamB	-
CF60_RS02090	438689..439030	+	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
CF60_RS02095	439242..439493	+	252	WP_001223206.1	type II toxin-antitoxin system ChpS family antitoxin	Antitoxin
CF60_RS02100	439487..439837	+	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
CF60_RS02105	439916..440446	-	531	WP_000055075.1	inorganic diphosphatase	-
CF60_RS02110	440756..441712	+	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
CF60_RS02115	441845..443347	+	1503	WP_001577603.1	sugar ABC transporter ATP-binding protein	-
CF60_RS02120	443361..444383	+	1023	WP_001316039.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T46378 WP_000239581.1 NZ_CP007394:439487-439837 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T46378 NZ_CP007394:439487-439837 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9303.62 Da
• Isoelectric Point: 4.6509

>AT46378 WP_001223206.1 NZ_CP007394:439242-439493 [Escherichia coli]
MRITIKKWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT46378 NZ_CP007394:439242-439493 [Escherichia coli]
ATGCGTATTACCATAAAAAAATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: V0YZQ7