Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3891428..3892082 Replicon chromosome
Accession NZ_CP007393
Organism Escherichia coli strain ST2747

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag CF59_RS18785 Protein ID WP_000244781.1
Coordinates 3891428..3891835 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag CF59_RS18790 Protein ID WP_000354046.1
Coordinates 3891816..3892082 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CF59_RS18765 3887385..3889118 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
CF59_RS18770 3889124..3889834 - 711 WP_025210838.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CF59_RS18775 3889859..3890755 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
CF59_RS18780 3890867..3891388 + 522 WP_001055874.1 flavodoxin FldB -
CF59_RS18785 3891428..3891835 - 408 WP_000244781.1 protein YgfX Toxin
CF59_RS18790 3891816..3892082 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
CF59_RS18795 3892325..3893305 + 981 WP_001587401.1 tRNA-modifying protein YgfZ -
CF59_RS18800 3893382..3894041 - 660 WP_000250274.1 hemolysin III family protein -
CF59_RS18805 3894205..3894516 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
CF59_RS18810 3894561..3895994 + 1434 WP_025210839.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T46373 WP_000244781.1 NZ_CP007393:c3891835-3891428 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T46373 NZ_CP007393:c3891835-3891428 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATCTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATCCACGGGTTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT46373 WP_000354046.1 NZ_CP007393:c3892082-3891816 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT46373 NZ_CP007393:c3892082-3891816 [Escherichia coli]
ATGGATATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAACCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References