Detailed information of TA system TA046361

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 2185428..2186029
• Replicon: chromosome
• Accession: NZ_CP007393
• Organism: Escherichia coli strain ST2747

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: CF59_RS10555
• Protein ID: WP_025210454.1
• Coordinates: 2185428..2185808 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: CF59_RS10560
• Protein ID: WP_001190712.1
• Coordinates: 2185808..2186029 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CF59_RS10530	2180869..2182353	-	1485	WP_000124159.1	hypothetical protein	-
CF59_RS10535	2182353..2183546	-	1194	WP_025210451.1	hypothetical protein	-
CF59_RS10540	2183632..2184084	-	453	WP_001326849.1	late promoter-activating protein	-
CF59_RS10545	2184173..2185216	-	1044	WP_025210452.1	DUF968 domain-containing protein	-
CF59_RS10550	2185244..2185423	-	180	WP_025210453.1	hypothetical protein	-
CF59_RS10555	2185428..2185808	-	381	WP_025210454.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
CF59_RS10560	2185808..2186029	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CF59_RS10565	2186212..2187768	+	1557	WP_025210455.1	type I restriction-modification system subunit M	-
CF59_RS10570	2187765..2188976	+	1212	WP_025210456.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2091722..2240084	148362

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (3-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13574.27 Da
• Isoelectric Point: 5.1514

>T46361 WP_025210454.1 NZ_CP007393:c2185808-2185428 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADSLRRLYGSAE

Nucleotide

• Length: 381 bp

>T46361 NZ_CP007393:c2185808-2185428 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCTGGCATGTC
AGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACTCGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT46361 WP_001190712.1 NZ_CP007393:c2186029-2185808 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT46361 NZ_CP007393:c2186029-2185808 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Antitoxin

• AlphaFold DB: A0A829CJB6