Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1135552..1136170 Replicon chromosome
Accession NZ_CP007393
Organism Escherichia coli strain ST2747

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag CF59_RS05390 Protein ID WP_001291435.1
Coordinates 1135552..1135770 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag CF59_RS05395 Protein ID WP_000344800.1
Coordinates 1135796..1136170 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CF59_RS05360 1130841..1131413 + 573 WP_000779826.1 lipoprotein -
CF59_RS05365 1131444..1131755 - 312 WP_000409911.1 MGMT family protein -
CF59_RS05370 1132134..1132487 + 354 WP_000878140.1 DUF1428 domain-containing protein -
CF59_RS05375 1132529..1134079 - 1551 WP_001389864.1 cyclic-guanylate-specific phosphodiesterase PdeB -
CF59_RS05380 1134243..1134713 - 471 WP_000136192.1 YlaC family protein -
CF59_RS05385 1134829..1135380 - 552 WP_025210255.1 maltose O-acetyltransferase -
CF59_RS05390 1135552..1135770 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
CF59_RS05395 1135796..1136170 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
CF59_RS05400 1136716..1139865 - 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
CF59_RS05405 1139888..1141081 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T46352 WP_001291435.1 NZ_CP007393:c1135770-1135552 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T46352 NZ_CP007393:c1135770-1135552 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT46352 WP_000344800.1 NZ_CP007393:c1136170-1135796 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT46352 NZ_CP007393:c1136170-1135796 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References