Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 341245..341742 Replicon chromosome
Accession NZ_CP007393
Organism Escherichia coli strain ST2747

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag CF59_RS01610 Protein ID WP_001173343.1
Coordinates 341569..341742 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A3W4RHQ7
Locus tag CF59_RS01605 Protein ID WP_001600874.1
Coordinates 341245..341541 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CF59_RS01585 337976..338695 - 720 WP_000611288.1 two-component system response regulator DcuR -
CF59_RS01590 338692..340323 - 1632 WP_001216477.1 sensor histidine kinase -
CF59_RS01595 340504..340734 + 231 WP_000373291.1 (4Fe-4S)-binding protein -
CF59_RS01600 340746..341018 + 273 WP_000405647.1 N-acetyltransferase -
CF59_RS01605 341245..341541 + 297 WP_001600874.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
CF59_RS01610 341569..341742 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
CF59_RS01615 341861..343378 - 1518 WP_025210125.1 lysine--tRNA ligase -
CF59_RS01620 343615..345072 - 1458 WP_000856848.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T46347 WP_001173343.1 NZ_CP007393:341569-341742 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T46347 NZ_CP007393:341569-341742 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11482.00 Da        Isoelectric Point: 4.1705

>AT46347 WP_001600874.1 NZ_CP007393:341245-341541 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRTLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT46347 NZ_CP007393:341245-341541 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGAACATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A3W4RHQ7

References