Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3896049..3896703 Replicon chromosome
Accession NZ_CP007392
Organism Escherichia coli strain ST2747

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag CF58_RS18830 Protein ID WP_000244781.1
Coordinates 3896049..3896456 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag CF58_RS18835 Protein ID WP_000354046.1
Coordinates 3896437..3896703 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CF58_RS18810 3892006..3893739 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
CF58_RS18815 3893745..3894455 - 711 WP_025210838.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CF58_RS18820 3894480..3895376 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
CF58_RS18825 3895488..3896009 + 522 WP_001055874.1 flavodoxin FldB -
CF58_RS18830 3896049..3896456 - 408 WP_000244781.1 protein YgfX Toxin
CF58_RS18835 3896437..3896703 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
CF58_RS18840 3896946..3897926 + 981 WP_001587401.1 tRNA-modifying protein YgfZ -
CF58_RS18845 3898003..3898662 - 660 WP_000250274.1 hemolysin III family protein -
CF58_RS18850 3898826..3899137 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
CF58_RS18855 3899182..3900615 + 1434 WP_025210839.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T46343 WP_000244781.1 NZ_CP007392:c3896456-3896049 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T46343 NZ_CP007392:c3896456-3896049 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATCTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATCCACGGGTTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT46343 WP_000354046.1 NZ_CP007392:c3896703-3896437 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT46343 NZ_CP007392:c3896703-3896437 [Escherichia coli]
ATGGATATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAACCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References