Detailed information of TA system TA046331

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 2184686..2185287
• Replicon: chromosome
• Accession: NZ_CP007392
• Organism: Escherichia coli strain ST2747

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: CF58_RS10585
• Protein ID: WP_025210454.1
• Coordinates: 2184686..2185066 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: CF58_RS10590
• Protein ID: WP_001190712.1
• Coordinates: 2185066..2185287 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CF58_RS10560	2180127..2181611	-	1485	WP_000124159.1	hypothetical protein	-
CF58_RS10565	2181611..2182804	-	1194	WP_025210451.1	hypothetical protein	-
CF58_RS10570	2182890..2183342	-	453	WP_001326849.1	late promoter-activating protein	-
CF58_RS10575	2183431..2184474	-	1044	WP_025210452.1	DUF968 domain-containing protein	-
CF58_RS10580	2184502..2184681	-	180	WP_025210453.1	hypothetical protein	-
CF58_RS10585	2184686..2185066	-	381	WP_025210454.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
CF58_RS10590	2185066..2185287	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CF58_RS10595	2185470..2187026	+	1557	WP_025210455.1	type I restriction-modification system subunit M	-
CF58_RS10600	2187023..2188234	+	1212	WP_025210456.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2084500..2246945	162445

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (3-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13574.27 Da
• Isoelectric Point: 5.1514

>T46331 WP_025210454.1 NZ_CP007392:c2185066-2184686 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADSLRRLYGSAE

Nucleotide

• Length: 381 bp

>T46331 NZ_CP007392:c2185066-2184686 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCTGGCATGTC
AGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACTCGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT46331 WP_001190712.1 NZ_CP007392:c2185287-2185066 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT46331 NZ_CP007392:c2185287-2185066 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Antitoxin

• AlphaFold DB: A0A829CJB6