Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 4060708..4061309 Replicon chromosome
Accession NZ_CP007391
Organism Escherichia coli strain ST540

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag CF61_RS20280 Protein ID WP_001216034.1
Coordinates 4060708..4061088 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag CF61_RS20285 Protein ID WP_001190712.1
Coordinates 4061088..4061309 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CF61_RS20255 4056639..4057571 - 933 WP_025269838.1 homocysteine S-methyltransferase -
CF61_RS20260 4057558..4058961 - 1404 WP_001373486.1 S-methylmethionine permease -
CF61_RS20265 4059169..4060185 + 1017 WP_012372828.1 IS5-like element IS5 family transposase -
CF61_RS20270 4060332..4060496 - 165 Protein_3859 pdcB -
CF61_RS20275 4060524..4060703 - 180 WP_001513661.1 hypothetical protein -
CF61_RS20280 4060708..4061088 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
CF61_RS20285 4061088..4061309 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
CF61_RS20290 4061492..4063048 + 1557 WP_025269840.1 type I restriction-modification system subunit M -
CF61_RS20295 4063045..4064259 + 1215 WP_025269841.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T46311 WP_001216034.1 NZ_CP007391:c4061088-4060708 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T46311 NZ_CP007391:c4061088-4060708 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCTTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT46311 WP_001190712.1 NZ_CP007391:c4061309-4061088 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT46311 NZ_CP007391:c4061309-4061088 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References