Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4081993..4082351 Replicon chromosome
Accession NZ_CP007275
Organism Escherichia coli strain O18

Toxin (Protein)


Gene name ccdB Uniprot ID -
Locus tag NMECO18_RS27160 Protein ID WP_001332330.1
Coordinates 4082229..4082351 (+) Length 41 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag NMECO18_RS20355 Protein ID WP_000125566.1
Coordinates 4081993..4082226 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NMECO18_RS20330 4077066..4077353 + 288 WP_000203741.1 ferredoxin-like protein FixX -
NMECO18_RS20335 4077412..4078743 + 1332 WP_001183207.1 MFS transporter -
NMECO18_RS20340 4078851..4079381 + 531 WP_000600749.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
NMECO18_RS20345 4079374..4081236 + 1863 WP_000377122.1 glutathione-regulated potassium-efflux system protein KefC -
NMECO18_RS20350 4081428..4081907 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
NMECO18_RS20355 4081993..4082226 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
NMECO18_RS27160 4082229..4082351 + 123 WP_001332330.1 CcdB family protein Toxin
NMECO18_RS27165 4082392..4082544 + 153 WP_146692624.1 CcdB family protein -
NMECO18_RS20365 4082541..4083389 - 849 WP_000257201.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
NMECO18_RS20370 4083396..4083773 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
NMECO18_RS20375 4083776..4084597 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
NMECO18_RS20380 4084594..4085583 - 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
NMECO18_RS20385 4085583..4086869 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-39)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 41 a.a.        Molecular weight: 4563.34 Da        Isoelectric Point: 10.9266

>T46267 WP_001332330.1 NZ_CP007275:4082229-4082351 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIVGEINRRIVIPLTPI

Download         Length: 123 bp

>T46267 NZ_CP007275:4082229-4082351 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTGTTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT46267 WP_000125566.1 NZ_CP007275:4081993-4082226 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT46267 NZ_CP007275:4081993-4082226 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References