Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2337935..2338589 Replicon chromosome
Accession NZ_CP007275
Organism Escherichia coli strain O18

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag NMECO18_RS11770 Protein ID WP_000244781.1
Coordinates 2337935..2338342 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag NMECO18_RS11775 Protein ID WP_000354046.1
Coordinates 2338323..2338589 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NMECO18_RS11750 2333892..2335625 - 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -
NMECO18_RS11755 2335631..2336341 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
NMECO18_RS11760 2336366..2337262 - 897 WP_000806987.1 site-specific tyrosine recombinase XerD -
NMECO18_RS11765 2337374..2337895 + 522 WP_001055874.1 flavodoxin FldB -
NMECO18_RS11770 2337935..2338342 - 408 WP_000244781.1 protein YgfX Toxin
NMECO18_RS11775 2338323..2338589 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
NMECO18_RS11780 2338832..2339812 + 981 WP_000886078.1 tRNA-modifying protein YgfZ -
NMECO18_RS11785 2339889..2340548 - 660 WP_000250274.1 hemolysin III family protein -
NMECO18_RS11790 2340712..2341023 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
NMECO18_RS11795 2341068..2342501 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T46262 WP_000244781.1 NZ_CP007275:c2338342-2337935 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T46262 NZ_CP007275:c2338342-2337935 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT46262 WP_000354046.1 NZ_CP007275:c2338589-2338323 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT46262 NZ_CP007275:c2338589-2338323 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References