Detailed information of TA system TA046209

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 496382..497019
• Replicon: chromosome
• Accession: NZ_CP007244
• Organism: Bacillus amyloliquefaciens subsp. plantarum TrigoCor1448

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: AJ82_RS02515
• Protein ID: WP_003156187.1
• Coordinates: 496669..497019 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: AJ82_RS02510
• Protein ID: WP_003156188.1
• Coordinates: 496382..496663 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AJ82_RS02490	492747..493346	-	600	WP_015239195.1	rhomboid family intramembrane serine protease	-
AJ82_RS02495	493439..493804	+	366	WP_007410229.1	holo-ACP synthase	-
AJ82_RS02500	493969..494976	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
AJ82_RS02505	495093..496262	+	1170	WP_025284272.1	alanine racemase	-
AJ82_RS02510	496382..496663	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
AJ82_RS02515	496669..497019	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
AJ82_RS02520	497137..497958	+	822	WP_003156182.1	STAS domain-containing protein	-
AJ82_RS02525	497963..498328	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
AJ82_RS02530	498331..498732	+	402	WP_003156178.1	anti-sigma regulatory factor	-
AJ82_RS02535	498744..499751	+	1008	WP_007410233.1	PP2C family protein-serine/threonine phosphatase	-
AJ82_RS02540	499815..500144	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
AJ82_RS02545	500141..500623	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
AJ82_RS02550	500589..501377	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
AJ82_RS02555	501377..501979	+	603	WP_007410234.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T46209 WP_003156187.1 NZ_CP007244:496669-497019 [Bacillus velezensis TrigoCor1448]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T46209 NZ_CP007244:496669-497019 [Bacillus velezensis TrigoCor1448]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT46209 WP_003156188.1 NZ_CP007244:496382-496663 [Bacillus velezensis TrigoCor1448]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT46209 NZ_CP007244:496382-496663 [Bacillus velezensis TrigoCor1448]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5