Detailed information of TA system TA046204

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1092987..1093904
• Replicon: chromosome
• Accession: NZ_CP007242
• Organism: Bacillus amyloliquefaciens KHG19

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: KHU1_RS05660
• Protein ID: WP_007407256.1
• Coordinates: 1093158..1093904 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: KHU1_RS05655
• Protein ID: WP_003154807.1
• Coordinates: 1092987..1093157 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KHU1_RS05620	1088221..1089843	+	1623	WP_042635123.1	pyocin knob domain-containing protein	-
KHU1_RS05625	1089856..1090227	+	372	WP_007407260.1	hypothetical protein	-
KHU1_RS05630	1090232..1090429	+	198	WP_007610833.1	XkdX family protein	-
KHU1_RS05635	1090486..1091247	+	762	WP_007407258.1	hypothetical protein	-
KHU1_RS05640	1091299..1091562	+	264	WP_003154815.1	hemolysin XhlA family protein	-
KHU1_RS05645	1091576..1091839	+	264	WP_003154813.1	phage holin	-
KHU1_RS05650	1091853..1092731	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
KHU1_RS05655	1092987..1093157	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
KHU1_RS05660	1093158..1093904	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
KHU1_RS05665	1094009..1095007	-	999	WP_020955696.1	inorganic phosphate transporter	-
KHU1_RS05670	1095020..1095637	-	618	WP_003154804.1	DUF47 domain-containing protein	-
KHU1_RS05675	1095923..1097239	-	1317	WP_007407254.1	amino acid permease	-
KHU1_RS05680	1097562..1098512	+	951	WP_007407253.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T46204 WP_007407256.1 NZ_CP007242:c1093904-1093158 [Bacillus amyloliquefaciens KHG19]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T46204 NZ_CP007242:c1093904-1093158 [Bacillus amyloliquefaciens KHG19]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGATTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTACTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT46204 WP_003154807.1 NZ_CP007242:c1093157-1092987 [Bacillus amyloliquefaciens KHG19]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT46204 NZ_CP007242:c1093157-1092987 [Bacillus amyloliquefaciens KHG19]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14