Detailed information of TA system TA046203

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 310158..310795
• Replicon: chromosome
• Accession: NZ_CP007242
• Organism: Bacillus amyloliquefaciens KHG19

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: KHU1_RS01450
• Protein ID: WP_003156187.1
• Coordinates: 310445..310795 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: KHU1_RS01445
• Protein ID: WP_003156188.1
• Coordinates: 310158..310439 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KHU1_RS01425	306523..307122	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
KHU1_RS01430	307215..307580	+	366	WP_007410229.1	holo-ACP synthase	-
KHU1_RS01435	307745..308752	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
KHU1_RS01440	308869..310038	+	1170	WP_032866888.1	alanine racemase	-
KHU1_RS01445	310158..310439	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
KHU1_RS01450	310445..310795	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
KHU1_RS01455	310913..311734	+	822	WP_003156182.1	STAS domain-containing protein	-
KHU1_RS01460	311739..312104	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
KHU1_RS01465	312107..312508	+	402	WP_003156178.1	anti-sigma regulatory factor	-
KHU1_RS01470	312520..313527	+	1008	WP_007410233.1	PP2C family protein-serine/threonine phosphatase	-
KHU1_RS01475	313591..313920	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
KHU1_RS01480	313917..314399	+	483	WP_003156173.1	anti-sigma B factor RsbW	-
KHU1_RS01485	314365..315153	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
KHU1_RS01490	315153..315755	+	603	WP_007410234.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T46203 WP_003156187.1 NZ_CP007242:310445-310795 [Bacillus amyloliquefaciens KHG19]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T46203 NZ_CP007242:310445-310795 [Bacillus amyloliquefaciens KHG19]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT46203 WP_003156188.1 NZ_CP007242:310158-310439 [Bacillus amyloliquefaciens KHG19]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT46203 NZ_CP007242:310158-310439 [Bacillus amyloliquefaciens KHG19]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5