Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 3489476..3490110 Replicon chromosome
Accession NZ_CP007230
Organism Yersinia similis strain Y_sim_228

Toxin (Protein)


Gene name hicA Uniprot ID A0A0T9QKF4
Locus tag BF17_RS15790 Protein ID WP_025383392.1
Coordinates 3489476..3489652 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A0T9QKE2
Locus tag BF17_RS15795 Protein ID WP_025383393.1
Coordinates 3489703..3490110 (+) Length 136 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BF17_RS15740 3484871..3485476 + 606 WP_025383384.1 recombination protein NinG -
BF17_RS15745 3485473..3485961 + 489 WP_025383385.1 antiterminator -
BF17_RS15750 3486224..3486655 - 432 WP_012303803.1 hypothetical protein -
BF17_RS15755 3486860..3487192 + 333 WP_025383386.1 phage holin, lambda family -
BF17_RS15760 3487179..3487583 + 405 WP_174407871.1 M15 family metallopeptidase -
BF17_RS25060 3487786..3488175 + 390 WP_025383389.1 hypothetical protein -
BF17_RS23935 3488535..3489152 + 618 WP_167330752.1 terminase small subunit -
BF17_RS15790 3489476..3489652 + 177 WP_025383392.1 type II toxin-antitoxin system HicA family toxin Toxin
BF17_RS15795 3489703..3490110 + 408 WP_025383393.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BF17_RS15800 3490166..3491770 + 1605 WP_025383394.1 terminase large subunit -
BF17_RS15805 3492023..3493555 + 1533 WP_038402629.1 DUF1073 domain-containing protein -
BF17_RS15810 3493512..3494393 + 882 WP_038402194.1 head morphogenesis protein SPP1 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - msbA / nueA / ompA 3347758..3494979 147221


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(79-131)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6649.82 Da        Isoelectric Point: 11.4350

>T46198 WP_025383392.1 NZ_CP007230:3489476-3489652 [Yersinia similis]
VKQREFQRWLAGQGAEFSNGTNHLKIFLNGKQTIMPRHPGKEIPEPLRKAILKQLGLK

Download         Length: 177 bp

>T46198 NZ_CP007230:3489476-3489652 [Yersinia similis]
GTGAAACAACGCGAGTTCCAGCGTTGGCTAGCGGGGCAAGGAGCTGAATTTTCGAATGGCACTAATCACTTGAAGATTTT
CTTAAACGGCAAGCAAACGATAATGCCAAGACATCCGGGGAAAGAAATACCGGAGCCTCTGAGAAAAGCGATTCTTAAGC
AACTTGGACTTAAATAA

Antitoxin


Download         Length: 136 a.a.        Molecular weight: 14798.92 Da        Isoelectric Point: 4.5129

>AT46198 WP_025383393.1 NZ_CP007230:3489703-3490110 [Yersinia similis]
MRYPIKFEHDETGWCISFPDIPEALTGGATKEEALSLAQDALVTSFDFYFEDQRPVPMPSTDGEEFIEVPASVAAKVLLL
NAMLATGTTPAELARRLGTRPQDVNRIVNLGHTTKIDTIEAALKALGKRLEINVL

Download         Length: 408 bp

>AT46198 NZ_CP007230:3489703-3490110 [Yersinia similis]
ATGCGATATCCAATAAAATTTGAGCATGACGAAACCGGGTGGTGTATATCGTTCCCGGATATACCAGAAGCATTAACTGG
CGGAGCTACAAAGGAAGAAGCGCTATCATTGGCGCAAGACGCCCTTGTAACGTCGTTTGATTTCTATTTTGAAGACCAGC
GCCCGGTACCCATGCCAAGCACAGACGGTGAGGAGTTTATCGAGGTGCCTGCAAGCGTGGCCGCCAAAGTGCTATTGCTT
AACGCTATGCTGGCTACAGGAACAACACCGGCAGAGCTTGCCCGTCGCCTGGGTACACGCCCGCAAGACGTTAACCGCAT
TGTTAATCTTGGGCACACGACAAAAATCGACACGATAGAAGCGGCGCTTAAGGCGCTAGGTAAGCGTTTAGAAATAAACG
TTCTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0T9QKF4

Antitoxin

Source ID Structure
AlphaFold DB A0A0T9QKE2

References