Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4179058..4179631 Replicon chromosome
Accession NZ_CP007215
Organism Kosakonia sacchari SP1

Toxin (Protein)


Gene name shpA Uniprot ID A0A1G4Y5H4
Locus tag C813_RS42720 Protein ID WP_017457810.1
Coordinates 4179350..4179631 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A1G4Y5V1
Locus tag C813_RS42715 Protein ID WP_017457811.1
Coordinates 4179058..4179363 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C813_RS42700 4175652..4177805 + 2154 WP_017457813.1 carbon starvation protein A -
C813_RS42705 4177859..4178062 + 204 WP_007373011.1 YbdD/YjiX family protein -
C813_RS42710 4178073..4179029 + 957 WP_017457812.1 GTPase -
C813_RS42715 4179058..4179363 - 306 WP_017457811.1 BrnA antitoxin family protein Antitoxin
C813_RS42720 4179350..4179631 - 282 WP_017457810.1 BrnT family toxin Toxin
C813_RS42725 4179767..4181137 - 1371 WP_017457809.1 NAD-dependent succinate-semialdehyde dehydrogenase -
C813_RS42730 4181710..4182225 + 516 WP_017457808.1 VOC family protein -
C813_RS42735 4182227..4183330 - 1104 WP_017457807.1 M20 peptidase aminoacylase family protein -
C813_RS42740 4183348..4183815 - 468 WP_017457806.1 YjiG family protein -
C813_RS42745 4183808..4184491 - 684 WP_017457805.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-86)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(40-93)


Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10928.36 Da        Isoelectric Point: 7.0746

>T46183 WP_017457810.1 NZ_CP007215:c4179631-4179350 [Kosakonia sacchari SP1]
MEFEWDATKAISNLRKHGIRFEDAVLVFDDPQHLSIQDRFENGEYRWQTLGLVHGVVIILVAHTIRFESGHEVVRIISAR
KADRKERSRYEHG

Download         Length: 282 bp

>T46183 NZ_CP007215:c4179631-4179350 [Kosakonia sacchari SP1]
ATGGAGTTCGAATGGGACGCAACAAAAGCGATTAGCAATCTGCGCAAGCACGGTATCCGCTTTGAAGATGCCGTATTGGT
TTTTGATGATCCGCAACATCTGTCGATACAAGACAGGTTTGAAAACGGCGAATATCGTTGGCAAACCCTCGGGCTGGTTC
ATGGCGTGGTCATTATTTTGGTCGCGCATACTATTCGTTTTGAAAGTGGGCACGAGGTCGTTCGTATTATCAGCGCGCGT
AAGGCTGACAGAAAGGAGAGGAGCCGTTATGAGCATGGTTAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11673.44 Da        Isoelectric Point: 8.5038

>AT46183 WP_017457811.1 NZ_CP007215:c4179363-4179058 [Kosakonia sacchari SP1]
MSMVKHKQGEMPVLNKQREAELQALADKPDDEIDYSDIPATEEALWADAVRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMMRDLQNKK

Download         Length: 306 bp

>AT46183 NZ_CP007215:c4179363-4179058 [Kosakonia sacchari SP1]
ATGAGCATGGTTAAACATAAGCAGGGCGAGATGCCTGTGCTCAATAAGCAGCGTGAAGCTGAACTTCAAGCACTGGCAGA
TAAACCAGACGATGAGATTGATTACAGCGATATTCCTGCGACGGAAGAGGCGCTTTGGGCAGATGCGGTTCGCGGTAAAT
TTTTCCGCCCTTTAAAAACGCAGGCATCGGTGCGTATTGATGCGGATGTTATGGAATGGCTGAAGCGGCCAGGAAAAGGG
TATCAGACCCGGCTCAACGCAATTTTACGCGAAGCGATGATGCGCGATTTGCAAAACAAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1G4Y5H4

Antitoxin

Source ID Structure
AlphaFold DB A0A1G4Y5V1

References