Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3685167..3685783 Replicon chromosome
Accession NZ_CP007215
Organism Kosakonia sacchari SP1

Toxin (Protein)


Gene name Hha Uniprot ID A0A1G4ZCB1
Locus tag C813_RS40460 Protein ID WP_017459990.1
Coordinates 3685565..3685783 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A1G4ZCB5
Locus tag C813_RS40455 Protein ID WP_017459991.1
Coordinates 3685167..3685541 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C813_RS40445 3680273..3681466 + 1194 WP_017459993.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
C813_RS40450 3681489..3684638 + 3150 WP_017459992.1 multidrug efflux RND transporter permease subunit -
C813_RS40455 3685167..3685541 + 375 WP_017459991.1 Hha toxicity modulator TomB Antitoxin
C813_RS40460 3685565..3685783 + 219 WP_017459990.1 hemolysin expression modulator Hha Toxin
C813_RS40465 3685860..3686426 + 567 WP_017459989.1 maltose O-acetyltransferase -
C813_RS40470 3686527..3687006 + 480 WP_017459988.1 YlaC family protein -
C813_RS40475 3686966..3688420 - 1455 WP_017459987.1 PLP-dependent aminotransferase family protein -
C813_RS40480 3688520..3689221 + 702 WP_017459986.1 GNAT family N-acetyltransferase -
C813_RS40485 3689218..3689358 - 141 WP_025263710.1 type B 50S ribosomal protein L36 -
C813_RS40490 3689362..3689622 - 261 WP_017459984.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8651.08 Da        Isoelectric Point: 7.9893

>T46182 WP_017459990.1 NZ_CP007215:3685565-3685783 [Kosakonia sacchari SP1]
MSEKPLTKIDYLMRLRRCQTIDTLERVIEKNKYELPDEELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Download         Length: 219 bp

>T46182 NZ_CP007215:3685565-3685783 [Kosakonia sacchari SP1]
ATGTCCGAAAAACCATTAACAAAAATTGATTATTTGATGCGTTTAAGACGCTGTCAGACAATTGATACTCTTGAACGTGT
AATTGAAAAAAATAAATACGAACTTCCCGACGAGGAACTCGCGGTGTTTTATTCAGCAGCGGATCATCGTCTGGCAGAAC
TGACCATGAATAAGCTTTATGACAAAATCCCCTCCTCCGTGTGGAAGTTTGTACGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14550.41 Da        Isoelectric Point: 5.8346

>AT46182 WP_017459991.1 NZ_CP007215:3685167-3685541 [Kosakonia sacchari SP1]
MDEYSPKRHDIAQLKFLCETLYHDCLVNLEESHHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIELIDEYL
DDTFMLFSSYGINTHDLQKWRKTGNKLFRSFVNVSKANPVSHSC

Download         Length: 375 bp

>AT46182 NZ_CP007215:3685167-3685541 [Kosakonia sacchari SP1]
ATGGACGAATACTCGCCGAAAAGGCATGATATCGCGCAGCTCAAATTTCTCTGCGAAACGTTGTACCATGATTGCCTTGT
TAACCTTGAAGAGAGCCATCATGGGTGGGTTAACGATCCTACATCAGCAATCAATTTGCAGTTAAACGAGCTGATAGAAC
ACATAGCCACTTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTGATTGAACTGATAGACGAATACCTC
GACGATACGTTTATGTTGTTCAGTAGCTATGGCATAAATACACACGACTTACAGAAATGGCGTAAAACAGGTAACAAATT
GTTCCGCAGTTTCGTCAATGTAAGCAAGGCTAATCCTGTTAGCCACTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1G4ZCB1

Antitoxin

Source ID Structure
AlphaFold DB A0A1G4ZCB5

References