Detailed information of TA system TA046162

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1552128..1552657
• Replicon: chromosome
• Accession: NZ_CP007208
• Organism: Staphylococcus xylosus strain HKUOPL8

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: BE24_RS07545
• Protein ID: WP_029377956.1
• Coordinates: 1552128..1552490 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A1D4YKY9
• Locus tag: BE24_RS07550
• Protein ID: WP_017722121.1
• Coordinates: 1552487..1552657 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE24_RS07525	1549146..1549916	-	771	WP_038678880.1	RNA polymerase sigma factor SigB	-
BE24_RS07530	1549891..1550373	-	483	WP_029377953.1	anti-sigma B factor RsbW	-
BE24_RS07535	1550375..1550701	-	327	WP_029377954.1	anti-sigma factor antagonist	-
BE24_RS07540	1550781..1551782	-	1002	WP_029377955.1	PP2C family protein-serine/threonine phosphatase	-
BE24_RS07545	1552128..1552490	-	363	WP_029377956.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BE24_RS07550	1552487..1552657	-	171	WP_017722121.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
BE24_RS07555	1552745..1553893	-	1149	WP_029377957.1	alanine racemase	-
BE24_RS07560	1554007..1554360	-	354	WP_029377958.1	holo-ACP synthase	-
BE24_RS07565	1554422..1554913	-	492	WP_029377959.1	PH domain-containing protein	-
BE24_RS07570	1554900..1556408	-	1509	WP_038678882.1	PH domain-containing protein	-
BE24_RS07575	1556401..1556880	-	480	WP_038678884.1	PH domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13539.75 Da
• Isoelectric Point: 10.3173

>T46162 WP_029377956.1 NZ_CP007208:c1552490-1552128 [Staphylococcus xylosus]
MMRRGDVYLADLSPVQGSEQGGIRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKSKYKLDKDSVILLEQIR
TLDKNRLKEKLTYLSEKKMKEVNTAIDISLGLHNIRNHKS

Nucleotide

• Length: 363 bp

>T46162 NZ_CP007208:c1552490-1552128 [Staphylococcus xylosus]
ATGATGAGAAGAGGAGACGTTTATTTAGCTGATTTATCACCAGTACAAGGGTCTGAACAAGGGGGAATTAGACCCGTCGT
TATTATACAAAACGATACTGGTAACAAATATAGTCCTACAGTAATTGTAGCTGCAATTACTGGTAGGATTAATAAAGCTA
AAATACCTACACATGTAGAGATAGAAAAAAGTAAATATAAACTTGATAAAGATTCTGTTATATTGTTAGAACAGATCAGA
ACTTTAGATAAAAATAGGCTAAAGGAAAAATTAACATATCTTTCTGAGAAAAAAATGAAAGAAGTTAATACTGCTATTGA
TATAAGTTTAGGATTACATAATATTAGGAATCATAAATCCTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6305.92 Da
• Isoelectric Point: 4.0121

>AT46162 WP_017722121.1 NZ_CP007208:c1552657-1552487 [Staphylococcus xylosus]
MASFNQSRNHSIEQLLKEGYSQMADLNLSLAAEAFPFECEACDCNEVYISSKNNNE

Nucleotide

• Length: 171 bp

>AT46162 NZ_CP007208:c1552657-1552487 [Staphylococcus xylosus]
ATGGCAAGTTTTAATCAAAGTAGAAATCATAGTATTGAACAACTTTTAAAAGAAGGCTATTCTCAAATGGCTGACTTAAA
TCTCTCCCTTGCAGCAGAAGCCTTTCCATTTGAATGTGAAGCTTGTGATTGCAATGAAGTTTACATTAGCTCTAAGAATA
ATAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A1D4YKY9