Detailed information of TA system TA046160

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 900300..900874
• Replicon: chromosome
• Accession: NZ_CP007205
• Organism: Pasteurella multocida subsp. multocida PMTB2.1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: AW43_RS04295
• Protein ID: WP_061406134.1
• Coordinates: 900590..900874 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: AW43_RS04290
• Protein ID: WP_061406133.1
• Coordinates: 900300..900593 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AW43_RS04270	895459..896550	-	1092	WP_005751571.1	L-ascorbate 6-phosphate lactonase	-
AW43_RS04275	896900..898690	+	1791	WP_061406132.1	PTS ascorbate-specific subunit IIBC	-
AW43_RS04280	898735..899202	+	468	WP_005726755.1	PTS sugar transporter subunit IIA	-
AW43_RS04285	899220..899897	+	678	WP_005726756.1	3-keto-L-gulonate-6-phosphate decarboxylase UlaD	-
AW43_RS04290	900300..900593	-	294	WP_061406133.1	putative addiction module antidote protein	Antitoxin
AW43_RS04295	900590..900874	-	285	WP_061406134.1	hypothetical protein	Toxin
AW43_RS04305	902152..902511	-	360	WP_095261631.1	DUF3418 domain-containing protein	-
AW43_RS04310	902655..903206	+	552	WP_196766247.1	NERD domain-containing protein	-
AW43_RS04315	903491..905080	+	1590	WP_081106270.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (4-87)

Antitoxin

• Uncharacterized protein HI1420 (7-92)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10667.40 Da
• Isoelectric Point: 10.2297

>T46160 WP_061406134.1 NZ_CP007205:c900874-900590 [Pasteurella multocida subsp. multocida PMTB2.1]
VGRLKEVKILSAKSAVLARINKAMNGNFGDHKSIGNGLYEMRIIKGSGYRVYYGQYREVTYLLICGGDKSTQKSDIVKAR
ELWKEIKQQEEVKV

Nucleotide

• Length: 285 bp

>T46160 NZ_CP007205:c900874-900590 [Pasteurella multocida subsp. multocida PMTB2.1]
GTGGGTCGGTTAAAGGAGGTAAAAATTTTATCCGCCAAATCTGCTGTTCTTGCTCGTATTAACAAAGCAATGAATGGTAA
TTTTGGCGATCATAAATCGATTGGAAATGGATTGTATGAGATGAGAATTATAAAAGGTTCGGGTTATAGAGTTTACTATG
GACAATATAGAGAAGTAACTTATTTATTAATTTGTGGTGGCGATAAATCAACACAGAAATCAGACATTGTTAAAGCGAGA
GAGTTGTGGAAAGAAATAAAGCAACAAGAGGAAGTAAAAGTATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10947.88 Da
• Isoelectric Point: 8.9149

>AT46160 WP_061406133.1 NZ_CP007205:c900593-900300 [Pasteurella multocida subsp. multocida PMTB2.1]
MSKKKIKLKLFDIAEHLDSEEMIAAYLSEVLETCDTNEFISALSDVVRAKGMTELAAKTGLRRESLYKTLSYGSKPRFDT
IMKIMHSLGIKLVPTHI

Nucleotide

• Length: 294 bp

>AT46160 NZ_CP007205:c900593-900300 [Pasteurella multocida subsp. multocida PMTB2.1]
ATGAGTAAGAAAAAAATAAAATTAAAACTGTTTGATATTGCTGAACATCTAGACAGTGAAGAAATGATTGCGGCATATTT
AAGTGAAGTTTTAGAAACATGTGATACTAATGAATTTATTTCCGCTTTAAGTGATGTTGTAAGAGCAAAAGGAATGACGG
AGTTAGCAGCAAAAACAGGGTTAAGAAGAGAAAGCCTCTATAAAACATTATCATACGGTAGCAAGCCACGTTTTGATACG
ATAATGAAAATTATGCATTCCTTAGGTATTAAACTTGTCCCAACGCACATCTAA